Jiang Wenwei, Han Jintao, Li Jianyuan, Yao Jin
Department of Traditional Chinese Medicine, Huzhou Central Hospital, Huzhou, Zhejiang Province, PR China.
Department of Drug Dispensing and Supply, Huzhou Central Hospital, Huzhou, Zhejiang Province, PR China.
Histol Histopathol. 2025 Jun 20:18955. doi: 10.14670/HH-18-955.
Menstrual stoppage, follicular dysplasia, and hypergonadotropic hypoestrogenism in women under forty are among the symptoms of premature ovarian failure (POF). This study aimed to explore the role and mechanism of amygdalin on ovarian function in a POF mouse model.
A POF mouse model was established via injection of D-galactose (D-gal), followed by amygdalin treatment. Histological staining of ovarian tissues was applied to determine follicular development and granulosa cell apoptosis. Levels of malondialdehyde (MDA), glutathione peroxidase (GSH-px), and superoxide dismutase (SOD) were measured in ovarian tissues. Enzyme-linked immunosorbent assay was used to detect levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), progesterone (P), estradiol (E2), anti-Müllerian hormone (AMH), and reactive oxygen species (ROS) in serum, and tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6 levels in ovaries.
D-gal increased levels of FSH, LH, ROS, MDA, TNF-α, IL-1β, IL-6, Bax, atretic follicles, and granulosa cell apoptosis, and decreased P, E2, AMH, SOD, GSH-px, Bcl-2, and primordial, primary, secondary, and total follicles (<0.01). Amygdalin with different concentrations reversed the effects of D-gal on mice (<0.05).
Amygdalin improved ovarian function in POF mice through inhibiting oxidative stress, inflammation, and granulosa cell apoptosis. These results suggested that amygdalin may be a potential antioxidant for POF treatment.
40岁以下女性出现月经停止、卵泡发育异常和高促性腺激素性低雌激素血症是卵巢早衰（POF）的症状。本研究旨在探讨苦杏仁苷对POF小鼠模型卵巢功能的作用及机制。
通过注射D-半乳糖（D-gal）建立POF小鼠模型，随后进行苦杏仁苷治疗。应用卵巢组织的组织学染色来确定卵泡发育和颗粒细胞凋亡情况。检测卵巢组织中丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-px）和超氧化物歧化酶（SOD）的水平。采用酶联免疫吸附测定法检测血清中卵泡刺激素（FSH）、黄体生成素（LH）、孕酮（P）、雌二醇（E2）、抗苗勒管激素（AMH）和活性氧（ROS）的水平，以及卵巢中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和IL-6的水平。
D-gal增加了FSH、LH、ROS、MDA、TNF-α、IL-1β、IL-6、Bax、闭锁卵泡和颗粒细胞凋亡的水平，降低了P、E2、AMH、SOD、GSH-px、Bcl-2以及原始卵泡、初级卵泡、次级卵泡和卵泡总数（<0.01）。不同浓度的苦杏仁苷可逆转D-gal对小鼠的影响（<0.05）。
苦杏仁苷通过抑制氧化应激、炎症反应和颗粒细胞凋亡改善了POF小鼠的卵巢功能。这些结果表明苦杏仁苷可能是一种用于POF治疗的潜在抗氧化剂。
