Wessendorf J, Rettenmaier H, Lingens F
Biol Chem Hoppe Seyler. 1985 Oct;366(10):945-51. doi: 10.1515/bchm3.1985.366.2.945.
From humus obtained from Stuttgart, a bacterium was isolated with lawsone (2-hydroxy-1,4-naphthoquinone) as selective source of carbon. This bacterium is capable of utilizing lawsone as sole source of carbon and energy. Morphological and physiological characteristics of the bacterium were examined and it was identified as a strain of Pseudomonas putida. The organism is referred to as Pseudomonas putida L2. The degradation of lawsone by Pseudomonas putida L2 was investigated. Salicylic acid and catechol were isolated and identified as metabolites. In lawsone-induced cells of Pseudomonas putida L2, salicylic acid is converted to catechol by salicylate 1-monooxygenase. Catechol 1,2-dioxygenase catalyses ortho-fission of catechol which is then metabolized via the beta-ketoadipate pathway. Formation of cis,cis-muconate and beta-ketoadipate was demonstrated by enzyme assays. Salicylate 1-monooxygenase and catechol 1,2-dioxygenase are induced sequentially. The enzymes of the beta-ketoadipate pathway are also inducible. Naphthoquinone hydroxylase, however, was demonstrated in induced and non-induced cells. This constitutive enzyme enables Pseudomonas putida L2 to degrade various 1,4-naphthoquinones in experiments with resting cells.
从斯图加特的腐殖土中分离出一种细菌，它能以胡桃醌（2-羟基-1,4-萘醌）作为碳的选择性来源。这种细菌能够利用胡桃醌作为唯一的碳源和能源。对该细菌的形态和生理特征进行了检测，并将其鉴定为恶臭假单胞菌菌株。该菌株被称为恶臭假单胞菌L2。研究了恶臭假单胞菌L2对胡桃醌的降解情况。分离并鉴定出了水杨酸和儿茶酚作为代谢产物。在恶臭假单胞菌L2的胡桃醌诱导细胞中，水杨酸通过水杨酸1-单加氧酶转化为儿茶酚。儿茶酚1,2-双加氧酶催化儿茶酚的邻位裂解，然后通过β-酮己二酸途径进行代谢。通过酶分析证实了顺,顺-粘康酸和β-酮己二酸的形成。水杨酸1-单加氧酶和儿茶酚1,2-双加氧酶是依次诱导产生的。β-酮己二酸途径的酶也是可诱导的。然而，在诱导细胞和未诱导细胞中均检测到萘醌羟化酶。这种组成型酶使恶臭假单胞菌L2在静息细胞实验中能够降解各种1,4-萘醌。
