Laboratory of Biosensor, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Center for Biological Research of the Russian Academy of Sciences, Pushchino, 142290 Moscow Region, Russia.
Laboratory of Microbial Enzymology, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Center for Biological Research of the Russian Academy of Sciences, Pushchino, 142290 Moscow Region, Russia.
Biosensors (Basel). 2021 Feb 21;11(2):56. doi: 10.3390/bios11020056.
Microbial reactor sensors (based on freshly harvested intact microbial cells) or microbial membrane sensors (based on immobilized microbial cells) can be used as convenient instruments for studying processes that cause the response of a biosensor, such as the properties of enzymes or the characteristics of metabolism. However, the mechanisms of the formation of biosensors responses have not yet been fully understood to study only one of these processes. In this work, the results of studies on the formation of a response to juglone for intact and immobilized bacterial cells used as receptors are presented. It was shown that the contribution of reactive oxygen species (ROS) to the formation of the biosensor response depends on the culture receptor and the form of juglone, quinone, or phenolate used. The response to the quinone form of juglone both for intact and immobilized cells of catalase-positive actinobacterium is formed regardless of the presence of ROS. The response of freshly harvested intact actinobacterial cells was caused by the rate of the enzymatic conversion of juglone. The rate of the response of immobilized actinobacterial cells was influenced by the activity of transport systems and metabolism. The response of immobilized pseudomonad cells was caused by the transport of juglone into cells, the inhibitory effect of juglone-induced ROS, and juglone metabolism.
微生物反应器传感器（基于新鲜收获的完整微生物细胞）或微生物膜传感器（基于固定化微生物细胞）可用作研究引起生物传感器响应的过程的便捷仪器，例如酶的性质或代谢特征。然而，仅研究这些过程中的一个过程，尚未完全了解生物传感器响应形成的机制。在这项工作中，介绍了作为受体的完整和固定化细菌细胞对胡桃醌形成响应的研究结果。结果表明，活性氧物质（ROS）对生物传感器响应形成的贡献取决于培养受体以及使用的胡桃醌、醌或酚盐的形式。对于过氧化氢酶阳性放线菌的完整和固定化细胞，醌形式的胡桃醌的响应的形成与 ROS 的存在无关。新鲜收获的完整放线菌细胞的响应是由胡桃醌的酶促转化速率引起的。固定化放线菌细胞的响应受到运输系统和代谢活性的影响。固定化假单胞菌细胞的响应是由胡桃醌进入细胞的运输、胡桃醌诱导的 ROS 的抑制作用以及胡桃醌代谢引起的。
