Umemori Miyaka, Tashiro Kojiro, Horiguchi Ayana, Urabe Fumihiko, Kimura Takahiro, Sato Shun, Hiroyuki Takahashi, Kurata Morito
Department of Clinical Laboratory Technology, Juntendo University, Chiba, Japan.
Department of Comprehensive Pathology, Institute of Science Tokyo, Tokyo, Japan.
Acta Histochem Cytochem. 2025 Jun 24;58(3):133-141. doi: 10.1267/ahc.25-00008. Epub 2025 Jun 18.
The long non-coding RNA reportedly forms a circular RNA variant (circ). As circ is expressed in various cancers and has been implicated in promoting cancer cell proliferation and tumor progression, it is considered a potential biomarker and therapeutic target. We previously confirmed that circ expression varies according to the Gleason pattern, a morphological indicator of malignancy in prostate cancer. In this study, we assessed the expression of circ using BaseScope assay with prostate cancer tissues and evaluated the correlation with the Grade Group (based on Gleason pattern), an indicator used to morphologically evaluate the degree of malignancy of prostate cancer. The relationship between circ expression and tumor proliferation was evaluated using cells in which circ expression was suppressed using the clustered regularly interspaced short palindromic repeats (CRISPR)/RfxCas13d system. BaseScope assay confirmed that circ expression was significantly higher in Grade Group 2-5 (intermediate- and high-grade groups) than Grade Group 1 (low-grade group). experiments using the CRISPR/RfxCas13d system showed that specific suppression of circ expression resulted in a significant reduction in the number of prostate cancer cells. The results of this study suggest that circ is involved in tumor growth in prostate cancer and may serve as a therapeutic target for moderately and highly malignant prostate cancers that express circ.
据报道，长链非编码RNA形成一种环状RNA变体（circ）。由于circ在多种癌症中表达，并与促进癌细胞增殖和肿瘤进展有关，因此它被认为是一种潜在的生物标志物和治疗靶点。我们之前证实，circ的表达根据Gleason模式而变化，Gleason模式是前列腺癌恶性程度的一种形态学指标。在本研究中，我们使用BaseScope检测法评估前列腺癌组织中circ的表达，并评估其与分级组（基于Gleason模式）的相关性，分级组是用于从形态学上评估前列腺癌恶性程度的指标。使用成簇规律间隔短回文重复序列（CRISPR）/RfxCas13d系统抑制circ表达的细胞来评估circ表达与肿瘤增殖之间的关系。BaseScope检测法证实，分级组2-5（中高级组）中的circ表达明显高于分级组1（低级组）。使用CRISPR/RfxCas13d系统进行的实验表明，特异性抑制circ表达会导致前列腺癌细胞数量显著减少。本研究结果表明，circ参与前列腺癌的肿瘤生长，对于表达circ的中度和高度恶性前列腺癌可能是一种治疗靶点。
