环状 RNA PVT1 作为竞争性内源性 RNA 促进 miR-497 促进非小细胞肺癌进展。
Circular RNA PVT1 acts as a competing endogenous RNA for miR-497 in promoting non-small cell lung cancer progression.
机构信息
Department of Respiration, The First Hospital of Jilin University, No. 71 Xinmin Street, Changchun, 130021, Jilin Province, China.
Shenzhen College of International Education, First HuangGang Park Street, Shenzhen 518048, Guangdong Province, China.
出版信息
Biomed Pharmacother. 2019 Mar;111:244-250. doi: 10.1016/j.biopha.2018.12.007. Epub 2018 Dec 24.
Circular RNAs (circRNAs) are a class of covalently closed non-coding RNAs and play crucial regulatory roles in human cancer biology. The purpose of the present study was to explore the expression pattern and biological role of circular RNA PVT1 (circPVT1) in non-small cell lung cancer (NSCLC). We firstly found that circPVT1 was overexpressed in clinical NSCLC tissues and cell lines. NSCLC patients with high expression of circPVT1 exhibited aggressive clinicopathological characteristics and poor prognosis. In vitro assays of the NSCLC cell lines (H1299 and A549 cells) demonstrated that knockdown of circPVT1 inhibited NSCLC cell proliferation and induced NSCLC cell apoptosis. We further found that circPVT1 served as a competing endogenous RNA of miR-497 and indirectly regulated Bcl-2 expression in NSCLC cells. Finally, inhibition of miR-497 abrogated the effects of circPVT1 knockdown in NSCLC cells. Taken together, the results from our study indicated circPVT1 acts as an oncogene in NSCLC, and may serve as a promising therapeutic target for NSCLC patients.
环状 RNA（circRNAs）是一类共价闭合的非编码 RNA，在人类癌症生物学中发挥着关键的调节作用。本研究旨在探讨环状 RNA PVT1（circPVT1）在非小细胞肺癌（NSCLC）中的表达模式和生物学作用。我们首先发现 circPVT1 在临床 NSCLC 组织和细胞系中表达上调。circPVT1 高表达的 NSCLC 患者表现出侵袭性临床病理特征和不良预后。在 NSCLC 细胞系（H1299 和 A549 细胞）的体外实验中，证实 circPVT1 的敲低抑制了 NSCLC 细胞的增殖并诱导了 NSCLC 细胞的凋亡。我们进一步发现 circPVT1 作为 miR-497 的竞争性内源 RNA，间接调节 NSCLC 细胞中 Bcl-2 的表达。最后，抑制 miR-497 消除了 circPVT1 敲低对 NSCLC 细胞的影响。综上所述，我们的研究结果表明 circPVT1 在 NSCLC 中作为癌基因发挥作用，可能成为 NSCLC 患者有前途的治疗靶点。
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