Chen J, Stuckey E C, Berry C L
Br J Exp Pathol. 1985 Oct;66(5):551-9.
Rat pancreatic cells were dissociated using a combined enzyme and EDTA method, grown on a plastic surface and then overlayed with collagen gel. Our studies have shown that exocrine pancreatic cells grown in this way have the ability to rearrange themselves into a three-dimensional organoid structure in which well defined epithelial lumina have been identified by ultrastructural and light microscopic examination. This in vitro system has advantages in examining the cytodifferentiation of pancreatic cells and may be exploited in studying pancreatic carcinogenesis.
使用酶和乙二胺四乙酸（EDTA）联合方法分离大鼠胰腺细胞，将其培养在塑料表面，然后覆盖胶原凝胶。我们的研究表明，以这种方式培养的胰腺外分泌细胞有能力重新排列形成三维类器官结构，通过超微结构和光学显微镜检查已确定其中有明确界定的上皮管腔。这种体外系统在研究胰腺细胞的细胞分化方面具有优势，可用于研究胰腺癌的发生。
