Hill R, Walker D M
Br J Nutr. 1985 May;53(3):587-93. doi: 10.1079/bjn19850068.
Eight newborn lambs were given milk-substitutes based on casein (n 4) or a soya-bean-protein isolate (n 4). On the morning before a 10 d collection period, the milk-substitutes were supplemented with 59Fe as ferric chloride and 65Zn as zinc chloride. Faeces and urine were collected daily and determinations of 59Fe and 65Zn were made on these separately. During a second 10 d period the dosing procedure and collection were repeated but the diet of each lamb was reversed. Mean retention of 59Fe in lambs given the casein diet was 0.50 (SE 0.12) and in those given the soya-bean-protein diet 0.06 (SE 0.05). There were large variations among animals but the treatment effect was significant at P less than 0.05. Retention of 65Zn was significantly greater than that of 59Fe for all lambs but the effect of dietary treatment was similar to that for Fe. 65Zn retention values for casein and soya-bean-protein diets were 0.84 (SE 0.06) and 0.52 (SE 0.03) respectively, and the difference was significant at P less than 0.01. There were only very small amounts of 59Fe and 65Zn in urine, representing 0.002-0.003 of the dose.
八只新生羔羊分别被给予基于酪蛋白的代乳品（4只）或大豆分离蛋白代乳品（4只）。在为期10天的收集期前一天早晨，代乳品中添加了氯化铁形式的59Fe和氯化锌形式的65Zn。每天收集粪便和尿液，并分别对其中的59Fe和65Zn进行测定。在第二个10天期间，重复给药程序和收集过程，但每只羔羊的饮食进行了对换。给予酪蛋白饮食的羔羊中59Fe的平均保留率为0.50（标准误0.12），给予大豆蛋白饮食的羔羊中为0.06（标准误0.05）。动物个体之间存在很大差异，但处理效应在P小于0.05时具有显著性。所有羔羊中65Zn的保留率显著高于59Fe，但饮食处理的效果与铁相似。酪蛋白和大豆蛋白饮食的65Zn保留值分别为0.84（标准误0.06）和0.52（标准误0.03），差异在P小于0.01时具有显著性。尿液中59Fe和65Zn的含量极少，仅占给药剂量的0.002 - 0.003。
