Dietrich C P, de Paiva J F, Moraes C T, Takahashi H K, Porcionatto M A, Nader H B
Biochim Biophys Acta. 1985 Nov 22;843(1-2):1-7. doi: 10.1016/0304-4165(85)90041-8.
The isolation, some structural features, physicochemical properties and pharmacological activities of a heparin from Anomalocardia brasiliana are reported. It is shown that the mollusc heparin is very similar to those present in mammalian tissues with regard to chemical composition, physicochemical properties, pharmacological activities and susceptibility to heparinase and heparitinase II from Flavobacterium heparinum, as well as to the types of products formed by the action of these enzymes. Three significant quantitative differences were observed for the mollusc heparin when compared with the ones from mammalian origin, namely, a higher degree of binding with antithrombin III (45%), higher molecular weight (27-43 kDa) and higher anticoagulant activity (320 I.U./mg). The possible biological role of heparin is discussed in view of the present findings.
报道了从巴西心蛤中分离出的一种肝素的一些结构特征、理化性质和药理活性。结果表明，就化学组成、理化性质、药理活性以及对来自肝素黄杆菌的肝素酶和硫酸乙酰肝素酶II的敏感性，以及这些酶作用形成的产物类型而言，软体动物肝素与哺乳动物组织中的肝素非常相似。与源自哺乳动物的肝素相比，软体动物肝素观察到三个显著的定量差异，即与抗凝血酶III的结合程度更高（45%）、分子量更高（27 - 43 kDa）和抗凝活性更高（320国际单位/毫克）。鉴于目前的研究结果，讨论了肝素可能的生物学作用。
