Aiuchi T, Matsunaga M, Nakaya K, Nakamura Y
Biochim Biophys Acta. 1985 Nov 22;843(1-2):20-4. doi: 10.1016/0304-4165(85)90044-3.
Effects of three probes for measuring membrane potential, tetraphenylphosphonium (TPP+), rhodamine 6G and 3,3'-dipropylthiocarbocyanine (diS-C3-(5)) on energy metabolism in synaptosomes were investigated. None of the three probes had any effect on lactate production in synaptosomes. TPP+ and rhodamine 6G did not inhibit the respiration of synaptosomes with pyruvate and succinate as exogenous substrate and were only weakly inhibitory with endogenous substrates. In contrast, diS-C3-(5) markedly inhibited the respiration of synaptosomes with glucose, pyruvate and endogenous substrates. All three probes reduced ATP content in synaptosomes and depolarized the membrane potential in synaptosomes with increasing concentrations of the probes. It is, therefore, preferable to estimate membrane potential with TPP+ or rhodamine 6G at their low concentrations where their effect on metabolism is negligible.
研究了三种用于测量膜电位的探针，即四苯基鏻（TPP+）、罗丹明6G和3,3'-二丙基硫代羰花青（diS-C3-(5)）对突触体能量代谢的影响。这三种探针均对突触体中的乳酸生成没有任何影响。TPP+和罗丹明6G不抑制以丙酮酸和琥珀酸作为外源底物时突触体的呼吸作用，而对以内源底物时的呼吸作用仅有微弱抑制。相比之下，diS-C3-(5)显著抑制以葡萄糖、丙酮酸和内源底物时突触体的呼吸作用。随着探针浓度增加，所有三种探针均降低了突触体中的ATP含量并使突触体的膜电位去极化。因此，最好在其对代谢影响可忽略不计的低浓度下使用TPP+或罗丹明6G来估计膜电位。
