Aiuchi T, Matsunaga M, Daimatsu T, Nakaya K, Nakamura Y
Biochim Biophys Acta. 1984 Apr 11;771(2):228-34. doi: 10.1016/0005-2736(84)90537-6.
Fluorescence changes of rhodamine 6G in synaptosomal suspension, which are correlated to changes in membrane potential in synaptosomes, were measured in the presence of various monosaccharides and organic acids. Addition of D-glucose, D-mannose, pyruvate and L-lactate hyperpolarized the membrane potential, whereas D-fructose, L-glucose, D-galactose, citrate, succinate and L-glutamate were without effect on the membrane potential. Hyperpolarization induced by D-glucose was inhibited by cytochalasin B, phloretin, iodoacetate, F- and 2-deoxy-D-glucose, but not inhibited by oligomycin or phlorizin. On the other hand, hyperpolarization induced by pyruvate was inhibited by alpha-cyanocinnamate or phloretin, but not inhibited by cytochalasin B or F-. Elimination of Na+ in physiological saline depressed hyperpolarization of membrane potential induced by addition of D-glucose, L-lactate or pyruvate. These results suggest that the activity of (Na+ + K+)-ATPase in plasma membranes of synaptosomes is increased by ATP formed by glycolysis, and that the accumulated K+ in synaptosomes hyperpolarizes the membrane potential.
在存在各种单糖和有机酸的情况下，测量了突触体悬浮液中罗丹明6G的荧光变化，这些变化与突触体膜电位的变化相关。添加D-葡萄糖、D-甘露糖、丙酮酸和L-乳酸会使膜电位超极化，而D-果糖、L-葡萄糖、D-半乳糖、柠檬酸盐、琥珀酸盐和L-谷氨酸对膜电位没有影响。细胞松弛素B、根皮素、碘乙酸盐、氟化物和2-脱氧-D-葡萄糖可抑制D-葡萄糖诱导的超极化，但寡霉素或根皮苷不能抑制。另一方面，α-氰基肉桂酸盐或根皮素可抑制丙酮酸诱导的超极化，但细胞松弛素B或氟化物不能抑制。生理盐水中去除Na+会降低添加D-葡萄糖、L-乳酸或丙酮酸诱导的膜电位超极化。这些结果表明，糖酵解形成的ATP会增加突触体质膜中（Na+ + K+）-ATP酶的活性，并且突触体中积累的K+会使膜电位超极化。
