Qurbani Karzan, Wsw Hevy, Khdhr Rahel, Hussein Safin, Ibrahim Bahra, Mahmood Avin, Hama Lanya, Ibrahim Frishta, Amiri Omid
Department of Biology, College of Science, University of Raparin, Ranya-Sulaymaniyah General Road, Ranya, Kurdistan Region, 46012, Iraq.
Research Center, Cihan University Sulaimaniya, Sulaymaniyah, Kurdistan Region, 46001, Iraq.
Sci Rep. 2025 Jul 9;15(1):24663. doi: 10.1038/s41598-025-99238-8.
Effective remediation is needed to reduce environmental and health threats from heavy metal contamination. This work examines the bioremediation capability of a new bacterial consortium from the polluted Tanjaro River: Pseudomonas putida pUoR_24 and Pasteurella aerogenes aUoR_24. Research will evaluate the consortium's ability to reduce copper (Cu), zinc (Zn), and nickel (Ni) concentrations in different environments. The study used microbiological methods to investigate metal tolerance, reduction efficiency, and growth optimization at varied temperatures, salinities, and pH levels. Results demonstrate the consortium's superior metal tolerance, with Minimum Inhibitory Concentrations (MICs) of 8 mM for Cu and 7 mM for Ni. Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) showed outstanding metal reduction rates of 84.78% for Cu, 91.27% for Zn, and 88.22% for Ni, exceeding those of individual strains. Based on Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray (EDX) analyses, Cu exhibited the highest weight percentage (3.7%), followed by Ni (0.5%), while Zn was undetectable, suggesting preferential sequestration of Cu and Ni by the consortium. The consortium also displayed robust growth across a wide range of temperatures (20-37°C), salinities (up to 4% NaCl), and pH levels (2-11). These findings show that microbial consortia can establish eco-friendly bioremediation solutions for heavy metal-contaminated settings.
需要进行有效的修复以减少重金属污染对环境和健康造成的威胁。这项工作研究了从受污染的坦贾罗河分离出的一种新型细菌联合体的生物修复能力：恶臭假单胞菌pUoR_24和产气巴斯德氏菌aUoR_24。研究将评估该联合体在不同环境中降低铜（Cu）、锌（Zn）和镍（Ni）浓度的能力。该研究采用微生物学方法，调查了在不同温度、盐度和pH值水平下的金属耐受性、还原效率和生长优化情况。结果表明该联合体具有卓越的金属耐受性，铜的最小抑菌浓度（MIC）为8 mM，镍为7 mM。电感耦合等离子体发射光谱法（ICP-OES）显示，铜、锌和镍的金属还原率分别高达84.78%、91.27%和88.22%，超过了单个菌株。基于扫描电子显微镜（SEM）和能量色散X射线（EDX）分析，铜的重量百分比最高（3.7%），其次是镍（0.5%），而未检测到锌，这表明该联合体对铜和镍具有优先螯合作用。该联合体在广泛的温度（20-37°C）、盐度（高达4% NaCl）和pH值水平（2-11）范围内也表现出强劲的生长能力。这些发现表明，微生物联合体可为重金属污染环境建立生态友好型生物修复解决方案。
