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对加拿大安大略省南部农业生态系统中的节肢动物进行代谢条形码分析。

Metabarcoding arthropods in agroecosystems in Southern Ontario, Canada.

作者信息

Steinke Dirk, Perez Kate Hj, Prosser Sean W J, Sones Jayme E, Agda Jireh Ra, deWaard Stephanie L, deWaard Jeremy R, Zakharov Evgeny V, Ratnasingham Sujeevan, Hebert Paul D N, Fryxell John

机构信息

Centre for Biodiversity Genomics, University of Guelph, Guelph, Canada Centre for Biodiversity Genomics, University of Guelph Guelph Canada.

University of Guelph, Guelph, Canada University of Guelph Guelph Canada.

出版信息

Biodivers Data J. 2025 Jun 19;13:e158459. doi: 10.3897/BDJ.13.e158459. eCollection 2025.

DOI:10.3897/BDJ.13.e158459
PMID:40635779
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12238963/
Abstract

BACKGROUND

Metabarcoding can generate large numbers of georeferenced occurrence data from bulk samples at low cost. Its integration into the practice of agricultural invertebrate biomonitoring currently lacks both standard methods and example datasets that allow the identification of potential challenges and uncertainties.

NEW INFORMATION

For this study, we gathered metabarcoding data of terrestrial arthropods from Malaise trap samples across sites in southern Ontario, spanning a gradient from high production, intensely farmed areas to alternative land use farms with varying amounts of natural restoration of marginal lands. The result is one of the largest datasets available for comparison of how agricultural practices influence arthropod biodiversity.

摘要

背景

元条形码技术能够以低成本从大量样本中生成大量带有地理参考的出现数据。目前,将其整合到农业无脊椎动物生物监测实践中既缺乏标准方法,也缺乏可用于识别潜在挑战和不确定性的示例数据集。

新信息

在本研究中,我们收集了安大略省南部各地马氏网诱捕样本中陆生节肢动物的元条形码数据,涵盖了从高产、集约化养殖区到具有不同程度边缘土地自然恢复的替代土地利用农场的梯度范围。该结果是可用于比较农业实践如何影响节肢动物生物多样性的最大数据集之一。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2ba/12238963/1ead86362241/bdj-13-e158459-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2ba/12238963/e49575934b0b/bdj-13-e158459-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2ba/12238963/e03da86e4c08/bdj-13-e158459-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2ba/12238963/1ead86362241/bdj-13-e158459-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2ba/12238963/e49575934b0b/bdj-13-e158459-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2ba/12238963/e03da86e4c08/bdj-13-e158459-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2ba/12238963/1ead86362241/bdj-13-e158459-g003.jpg

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本文引用的文献

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Getting the bugs out of AI: Advancing ecological research on arthropods through computer vision.从人工智能中获取信息:通过计算机视觉推进节肢动物生态学研究。
Ecol Lett. 2023 Jul;26(7):1247-1258. doi: 10.1111/ele.14239. Epub 2023 May 22.
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Fluctuating insect diversity, abundance and biomass across agricultural landscapes.农业景观中昆虫多样性、丰度和生物量的波动。
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Message in a Bottle-Metabarcoding enables biodiversity comparisons across ecoregions.瓶中信——代谢条码技术能够实现跨生态区的生物多样性比较。
Gigascience. 2022 Apr 28;11. doi: 10.1093/gigascience/giac040.
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Agricultural intensification and climate change are rapidly decreasing insect biodiversity.农业集约化和气候变化正在迅速减少昆虫生物多样性。
Proc Natl Acad Sci U S A. 2021 Jan 12;118(2). doi: 10.1073/pnas.2002548117.
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Validation of COI metabarcoding primers for terrestrial arthropods.用于陆生节肢动物的COI元条形码引物的验证
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Metabarcoding a diverse arthropod mock community.对多样的节肢动物模拟群落进行代谢条形码分析。
Mol Ecol Resour. 2019 May;19(3):711-727. doi: 10.1111/1755-0998.13008.
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Climate-driven declines in arthropod abundance restructure a rainforest food web.气候驱动的节肢动物丰度下降重塑了热带雨林食物网。
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Where have all the insects gone?所有的昆虫都去哪儿了?
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DNA barcodes from century-old type specimens using next-generation sequencing.利用新一代测序技术从百年历史的模式标本中获取DNA条形码
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