Dabritz Haydee A, Panditrao Mayuri V, Pandey Suchitra, Dover Nir, Barash Jason R, Khouri Jessica M, Read Jennifer S
Infant Botulism Treatment and Prevention Program, Infectious Diseases Laboratories Division, Center for Laboratory Sciences, Richmond, CA.
Research and Evaluation Branch, Office of Health Equity, California Department of Public Health, Richmond, CA.
J Pediatr Clin Pract. 2025 Jun 6;17:200152. doi: 10.1016/j.jpedcp.2025.200152. eCollection 2025 Sep.
We investigated if specific carbohydrate blood group antigens were associated with infant botulism (IB) among discordant twins and triplets.
Infants in the US and Canada from 2015 to 2022 who had laboratory-confirmed IB; were members of a multiple gestation birth; and had unaffected sibling(s), were eligible to participate. Blood specimens were tested for ABO, Lewis, H, and P1 carbohydrate blood antigens. and genes were sequenced to determine secretor and Lewis antigen status, respectively. In addition, characteristics of IB cases in the study were compared to the singleton US-Canadian IB cases from 2015 to 2022.
Parents of 14 of 26 IB cases who were twins or triplets with unaffected siblings consented to participate. No statistically significant associations between blood group antigens (ABO, Lewis, H, and P1) and IB were demonstrated. Twin cases in this study were significantly younger at onset of illness (median age 8.6 vs 17.3 weeks for the singleton US-Canadian IB cases, test < .001); and less likely to be fed exclusively breastmilk (14% for twin vs 63% for the US-Canadian IB cases) and more likely to be mixed-fed breast milk and formula (86% for twin vs 23% for the US-Canadian IB cases, Fisher exact test < .001).
In this first-of-its-kind small study of IB twin or triplet cases and their unaffected siblings, no carbohydrate blood group antigens were associated with IB. Future studies could compare the microbial composition of the gut microbiome of IB cases and uninfected twin siblings or the prevalence of ABO blood groups in IB cases vs population norms.
我们调查了特定碳水化合物血型抗原是否与异卵双胞胎和三胞胎中的婴儿肉毒中毒（IB）有关。
2015年至2022年在美国和加拿大的婴儿，其IB经实验室确诊；为多胎妊娠出生的成员；且有未受影响的兄弟姐妹，符合参与条件。对血液标本进行ABO、Lewis、H和P1碳水化合物血型抗原检测。分别对 和 基因进行测序以确定分泌型和Lewis抗原状态。此外，将本研究中IB病例的特征与2015年至2022年美国-加拿大单胎IB病例进行比较。
26例为双胞胎或三胞胎且有未受影响兄弟姐妹的IB病例中，14例的父母同意参与。未发现血型抗原（ABO、Lewis、H和P1）与IB之间存在统计学上的显著关联。本研究中的双胞胎病例发病时年龄显著更小（中位年龄8.6周，而美国-加拿大单胎IB病例为17.3周，检验 <.001）；纯母乳喂养的可能性较小（双胞胎为14%，而美国-加拿大IB病例为63%），混合喂养母乳和配方奶的可能性较大（双胞胎为86%，而美国-加拿大IB病例为23%，Fisher精确检验 <.001）。
在这项对IB双胞胎或三胞胎病例及其未受影响的兄弟姐妹进行的同类小型研究中，没有碳水化合物血型抗原与IB相关。未来的研究可以比较IB病例和未感染的双胞胎兄弟姐妹的肠道微生物群的微生物组成，或者IB病例中ABO血型的患病率与人群标准。
