Deng Yiqin, Xu Liwen, Yang Keng, Lin Ziyang, Zang Shujun, Jiang Kui, Li Wangdong, Ibrahim Nabil A, Ma Hongling, Cheng Changhong, Feng Juan
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.
Guangzhou Xinhailisheng Biotechnology Co., Ltd, Guangzhou, 510300, Guangdong, China.
Probiotics Antimicrob Proteins. 2025 Jul 10. doi: 10.1007/s12602-025-10652-w.
Probiotic Clostridium butyricum has been demonstrated to enhance the growth of spotted sea bass (Lateolabrax maculatus). However, the time reliable effect of C. butyricum application for L. maculatus growth promotion is poorly understood, limiting our ability to make informed decisions about when the C. butyricum should be added and why. To address this, we employed multidisciplinary approach to investigate the growth response (including growth performance, intestinal morphology, digestive enzyme activities, and gut microbiome) of L. maculatus to C. butyricum supplementation throughout the entire breeding cycle (from juvenile to marketable size). The C. butyricum supplementation significantly increased the weight of L. maculatus during its early life stages. Correspondingly, 16S rRNA sequence showed that C. butyricum addition increased probiotic bacteria (including Lactobacillales), decreased pathogenic bacteria (including Enterobacterales, Burkholderiales, and Fusobacteriales), and reduced bacterial virulence processes (including quorum sensing, biofilm formation, and bacterial secretion systems) of gut microbiota in L. maculatus. Concurrently, C. butyricum supplementation increased intestinal villus height (up to 32.1%), muscle thickness (up to 115.8%), and digestive enzyme activities (up to 77.8%). Nevertheless, during the later stages of incubation, treatment with C. butyricum did not result in further improvements or even showed similar outcomes to the non-supplemented treatment. These findings emphasize that C. butyricum supplementation during the early breeding stage can significantly benefit L. maculatus growth, rather than adding it during later stages. This study provides evidence on the appropriate timing for C. butyricum supplementation in L. maculatus culture, offering timely information and technical support for its culture management.
已证明益生菌丁酸梭菌可促进花鲈（Lateolabrax maculatus）的生长。然而，人们对丁酸梭菌促进花鲈生长的可靠作用时间了解甚少，这限制了我们在何时添加丁酸梭菌以及为何添加方面做出明智决策的能力。为了解决这一问题，我们采用多学科方法，研究了花鲈在整个养殖周期（从幼鱼到上市规格）中对添加丁酸梭菌的生长反应（包括生长性能、肠道形态、消化酶活性和肠道微生物群）。在花鲈幼鱼阶段，添加丁酸梭菌显著增加了其体重。相应地，16S rRNA序列显示，添加丁酸梭菌增加了益生菌（包括乳杆菌目），减少了病原菌（包括肠杆菌目、伯克霍尔德菌目和梭杆菌目），并降低了花鲈肠道微生物群的细菌毒力过程（包括群体感应、生物膜形成和细菌分泌系统）。同时，添加丁酸梭菌增加了肠绒毛高度（高达32.1%）、肌肉厚度（高达115.8%）和消化酶活性（高达77.8%）。然而，在孵化后期，用丁酸梭菌处理并没有带来进一步改善，甚至与未添加处理的结果相似。这些发现强调，在养殖早期阶段添加丁酸梭菌可显著促进花鲈生长，而不是在后期添加。本研究为花鲈养殖中添加丁酸梭菌的适宜时机提供了证据，为其养殖管理提供了及时的信息和技术支持。
