Kong Lumin, Ma Jianrong, Zhou Sishun, Lin Hao, Long Zhongying, Qin Huihui, Liu Longhui, Lin Yi, Huang Zhangfan, Li Zhongbao
Fisheries College, Jimei University, Xiamen, China.
Fisheries College of Jimei University, Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China.
Front Immunol. 2023 Jul 14;14:1181471. doi: 10.3389/fimmu.2023.1181471. eCollection 2023.
(CB) is known to promote growth, enhance immunity, promote digestion, and improve intestinal health. In this study, we investigated the effects of CB in the feed on growth performance, digestion, and intestinal health of juvenile spotted sea bass. To provide a theoretical basis for the development and application of CB in the feed of spotted sea bass, a total of 450 spotted sea bass with an initial body weight of (9.58 ± 0.05) g were randomly divided into six groups. Gradient levels with 0, 0.1%, 0.2%, 0.3%, 0.4%, and 0.5% of CB (1×10 cfu/g) were supplemented into diets, designated as CC, CB1, CB2, CB3, CB4, and CB5, respectively. Each group was fed for 54 days. Our results suggest that dietary 0.2% and 0.3% of CB can significantly increase the weight gain (WG) and specific growth rate (SGR) of spotted sea bass. The addition of CB significantly increased intestinal amylase activity, intestinal villus length, intestinal villus width, and intestinal muscle thickness. Similarly, CB supplementation increased the expression of tumor necrosis factor- () and interleukin-8 (). Sequence analysis of the bacterial 16S rDNA region showed that dietary CB altered the intestinal microbiota profile of juvenile spotted sea bass, increasing the dominant bacteria in the intestine and decreasing the harmful bacteria. Overall, dietary addition of CB can improve growth performance, enhance intestinal immunity, improve intestinal flora structure, and comprehensively improve the health of spotted sea bass.
已知复合益生菌（CB）可促进生长、增强免疫力、促进消化并改善肠道健康。在本研究中，我们调查了饲料中CB对幼体花鲈生长性能、消化和肠道健康的影响。为给CB在花鲈饲料中的开发和应用提供理论依据，将450尾初始体重为（9.58±0.05）克的花鲈随机分为六组。分别在饲料中添加0、0.1%、0.2%、0.3%、0.4%和0.5%的CB（1×10 cfu/g）梯度水平，分别命名为CC、CB1、CB2、CB3、CB4和CB5。每组投喂54天。我们的结果表明，饲料中添加0.2%和0.3%的CB可显著提高花鲈的增重（WG）和特定生长率（SGR）。添加CB显著提高了肠道淀粉酶活性、肠绒毛长度、肠绒毛宽度和肠肌厚度。同样，添加CB增加了肿瘤坏死因子-（）和白细胞介素-8（）的表达。细菌16S rDNA区域的序列分析表明，饲料中的CB改变了幼体花鲈的肠道微生物群谱，增加了肠道中的优势菌并减少了有害菌。总体而言，饲料中添加CB可改善生长性能、增强肠道免疫力、改善肠道菌群结构，并全面改善花鲈的健康状况。
