de Barros Amanda Vieira, de Veras Bruno Oliveira, Costa Júnior Henrique Nelson Pereira, Mendes Raudiney Frankilin Vasconcelos, Santos Patryck Érmerson Monteiro Dos, Tavares Jael Fernandes, Navarro Daniela Maria do Amaral Ferraz, Ximenes Rafael Matos, Paiva Patrícia Maria Guedes, Bezerra Filho Clovis Macedo, da Silva Josinaldo Alves, da Silva Márcia Vanusa, de Lima João Paulo Martins, Coutinho Henrique Douglas Melo, de Oliveira Maria Betânia Melo
Department of Biochemistry, Federal University of Pernambuco, Recife, Brazil.
Department of Antibiotics, Federal University of Pernambuco, Recife, Brazil.
Chem Biodivers. 2025 Nov;22(11):e01177. doi: 10.1002/cbdv.202501177. Epub 2025 Jul 10.
Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are difficult to treat due to its resistance profile and the scarcity of new antibiotics, necessitating alternative therapeutic strategies. This study explores the antibacterial, antivirulence, and in vivo effects of Algrizea minor essential oil (EOAM) against MRSA isolates. Chemical analysis identified β-pinene (38.73%) and α-pinene (11.79%) as the major constituents of EOAM. The oil exhibited synergistic effects when combined with amoxicillin and oxacillin, inhibiting biofilm formation and reducing S. aureus-induced hemolysis. Additionally, EOAM enhanced the efficacy of hydrogen peroxide (H₂O₂) against S. aureus. In vivo, EOAM significantly increased the survival of Galleria mellonella larvae infected with MRSA, reducing bacterial load and myelinization. These findings highlight the therapeutic potential of EOAM in treating MRSA infections by modulating bacterial resistance and virulence, suggesting their potential for application in clinical settings.
耐甲氧西林金黄色葡萄球菌（MRSA）引起的感染因其耐药性和新型抗生素的稀缺而难以治疗，因此需要替代治疗策略。本研究探讨了小阿尔格里泽亚精油（EOAM）对MRSA分离株的抗菌、抗毒力及体内作用。化学分析确定β-蒎烯（38.73%）和α-蒎烯（11.79%）为EOAM的主要成分。该精油与阿莫西林和苯唑西林联合使用时表现出协同作用，抑制生物膜形成并减少金黄色葡萄球菌诱导的溶血。此外，EOAM增强了过氧化氢（H₂O₂）对金黄色葡萄球菌的功效。在体内，EOAM显著提高了感染MRSA的大蜡螟幼虫的存活率，降低了细菌载量并减少了髓鞘形成。这些发现突出了EOAM通过调节细菌耐药性和毒力来治疗MRSA感染的治疗潜力，表明其在临床环境中的应用潜力。
