Zhan Lei, Zhang Boqing, Wo Hongyun, Wu Lina, Li Huan, Liu Min, Li Zhuangzhuang, Chen Tao, Gui Xingyu, Wang Kaixin, Pu Xiaobing, Zhou Changchun
West China School of Public Health and West China Fourth Hospital, Sichuan Univetsity, Chengdu 610064, China.
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China; College of Biomedical Engineering, Sichuan University, Chengdu 610064, China.
Biomater Adv. 2025 Dec;177:214404. doi: 10.1016/j.bioadv.2025.214404. Epub 2025 Jul 7.
The aging population and the increasing prevalence of sports-related injuries have made osteochondral defects a major global health issue. The repair of osteochondral defect is still a challenge due to poor regeneration of both cartilage and bone tissues. To address this issue, this study developed a biomimetic dual-layer scaffold based on SilMA hydrogel using DLP technology. For the upper area of scaffold, PEGDA was used to enhance the mechanical strength of SilMA hydrogel, making it more suitable for the environment of articular cartilage, while cell microspheres were incorporated as 3D carriers for chondrocyte encapsulation and delivery, providing seed cells for regeneration. For the lower area of scaffold, the lower part was loaded with hydroxyapatite to promote bone tissue regeneration. This study systematically investigated the design of 3D printable inks for osteochondral defect repair, exploring the effects of PEGDA and HAp on the printability and physicochemical properties of SilMA bioink through orthogonal experiments. In vitro and in vivo results demonstrated that the biomimetic dual-layer scaffold exhibited good biocompatibility, promoting cell adhesion and proliferation. Thus, these dual-layer scaffolds loaded with chondrocyte microspheres effectively promote the synchronous repair of both articular cartilage and subchondral bone.
人口老龄化以及与运动相关损伤的患病率不断上升，使得骨软骨缺损成为一个重大的全球健康问题。由于软骨和骨组织的再生能力较差，骨软骨缺损的修复仍然是一项挑战。为了解决这个问题，本研究利用数字光处理（DLP）技术开发了一种基于SilMA水凝胶的仿生双层支架。对于支架的上部区域，使用聚乙二醇二丙烯酸酯（PEGDA）来增强SilMA水凝胶的机械强度，使其更适合关节软骨的环境，同时将细胞微球作为软骨细胞包封和递送的三维载体，为再生提供种子细胞。对于支架的下部区域，下部负载羟基磷灰石以促进骨组织再生。本研究系统地研究了用于骨软骨缺损修复的三维可打印墨水的设计，通过正交实验探索了PEGDA和羟基磷灰石（HAp）对SilMA生物墨水的可打印性和物理化学性质的影响。体外和体内结果表明，仿生双层支架表现出良好的生物相容性，促进细胞粘附和增殖。因此，这些负载软骨细胞微球的双层支架有效地促进了关节软骨和软骨下骨的同步修复。
