Jin Nana, Yang Fengzhen, Zhang Xinyang, Li Yanbin, Lin Jianhan
Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing, 100083, China; Xianghu Laboratory, Hangzhou, 311231, China.
Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing, 100083, China.
Biosens Bioelectron. 2025 Nov 1;287:117750. doi: 10.1016/j.bios.2025.117750. Epub 2025 Jul 4.
Foodborne pathogens, such as Salmonella typhimurium, E. coli O157:H7 and Listeria monocytogenes, have seriously threatened human health. To address the issues of traditional bacterial detection methods, such as long time, low sensitivity and complex operation, a versatile finger driven microfluidic chip was elaboratively developed and combined with recombinase aided amplification for fast, sensitive and simple detection of multiplex bacteria. In the microfluidic chip, three pneumatic check valves were designed to coordinate with their respective air chambers for transferring their corresponding solutions to their designated destinations, and a 3D-printed rotating valve was developed to select the designated solution to flow towards the designated direction. Besides, a portable device with a LED light as excitation source was developed to detect the fluorescent signals for bacterial quantification. Under the optimal conditions, the microfluidic chip could detect Salmonella typhimurium, E. coli O157:H7 and Listeria monocytogenes as low as 15 CFU/mL, 48 CFU/mL and 38 CFU/mL within 45 min. It was featured with simple operation, high sensitivity and short time, and showed the potential for in-field screening of foodborne pathogens to enhance food safety.
食源性病原体，如鼠伤寒沙门氏菌、大肠杆菌O157:H7和单核细胞增生李斯特菌，已严重威胁人类健康。为解决传统细菌检测方法存在的检测时间长、灵敏度低和操作复杂等问题，精心研制了一种通用的手指驱动微流控芯片，并将其与重组酶辅助扩增技术相结合，用于快速、灵敏且简便地检测多种细菌。在微流控芯片中，设计了三个气动止回阀，使其与各自的气室协同工作，将相应的溶液转移至指定位置，还开发了一个3D打印旋转阀，用于选择指定溶液流向指定方向。此外，还研发了一种以LED灯作为激发源的便携式设备，用于检测荧光信号以实现细菌定量。在最佳条件下，该微流控芯片能够在45分钟内检测到低至15 CFU/mL的鼠伤寒沙门氏菌、48 CFU/mL的大肠杆菌O157:H7和38 CFU/mL的单核细胞增生李斯特菌。其具有操作简单、灵敏度高和检测时间短的特点，显示出在现场筛查食源性病原体以提高食品安全方面的潜力。
