Wei Hui, Zhang Yi, Li Jiwei, Su Yu, Leng Yuankui, Xiong Yonghua, Huang Xiaolin
State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang 330047, PR China.
State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang 330047, PR China; Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, PR China.
Food Chem. 2025 Oct 15;489:144951. doi: 10.1016/j.foodchem.2025.144951. Epub 2025 May 27.
Herein, we introduce a novel lighting-up fluorescence immunoassay, designated as d-AIENPs-LFIA, for the synchronous detection of Escherichia coli O157:H7 and Salmonella typhimurium utilizing ultrabright red-emitting aggregation-induced emission nanoparticles (AIENPs) as signal-amplification probes in a lateral flow immunoassay (LFIA). Under the developed conditions, the d-AIENPs-LFIA demonstrated exceptional sensitivity, enabling highly sensitive synchronous detection of E. coli O157:H7 and S. typhimurium within 15 min, with detection limits of 396 and 236 CFU/mL, respectively. This d-AIENPs-LFIA exhibited good reproducibility, with average recoveries of 83.7 % to 113.0 % and coefficients of variation below 15.83 %. Furthermore, the practical applicability of the d-AIENPs-LFIA was further demonstrated in complex food matrices, including lettuce, skim milk powder, and milk samples. Notably, by incorporating a pre-incubation step, the sensitivity of the d-AIENPs-LFIA was significantly enhanced to 1 CFU/mL within 6 h, offering substantial potential for routine rapid screening of foodborne pathogens.
在此，我们介绍一种新型的点亮荧光免疫分析法，称为d-AIENPs-LFIA，用于在侧向流动免疫分析法（LFIA）中利用超亮红色发射聚集诱导发光纳米颗粒（AIENPs）作为信号放大探针同步检测大肠杆菌O157:H7和鼠伤寒沙门氏菌。在优化的条件下，d-AIENPs-LFIA表现出卓越的灵敏度，能够在15分钟内对大肠杆菌O157:H7和鼠伤寒沙门氏菌进行高灵敏度同步检测，检测限分别为396和236 CFU/mL。这种d-AIENPs-LFIA具有良好的重现性，平均回收率为83.7%至113.0%，变异系数低于15.83%。此外，d-AIENPs-LFIA在包括生菜、脱脂奶粉和牛奶样品在内的复杂食品基质中的实际适用性得到了进一步证明。值得注意的是，通过加入预孵育步骤，d-AIENPs-LFIA的灵敏度在6小时内显著提高到1 CFU/mL，为食源性病原体的常规快速筛查提供了巨大潜力。
