Yazaki K, Igarashi M, Wakabayashi K, Koide S S
Cancer Lett. 1985 Oct;29(1):23-8. doi: 10.1016/0304-3835(85)90118-1.
Human chorionic gonadotropin (hCG) in sera and placentae from normal pregnant women separated into 7 variants on analysis by an isoelectric focusing technique and determined by radioimmunoassay. The pIs ranged from 3.9 to 7.0. Three additional acidic variants were found in the sera and tumor tissues of patients with choriocarcinoma with pIs of 3.2, 3.5 and 3.7. The biological activity of each variant was determined by measuring testosterone production by rat Leydig cells in vitro. The pI 4.1 fraction corresponding to placental hCG possessed the highest biological activity while those focusing further afield from pI 4.1 showed decreasing activities. All 3 tumor unique acidic variants possessed biological activity with the fraction focusing at pI 3.7 having the greatest potency.
采用等电聚焦技术分析并通过放射免疫测定法测定，正常孕妇血清和胎盘中的人绒毛膜促性腺激素（hCG）可分离出7种变体。其等电点范围为3.9至7.0。在绒毛膜癌患者的血清和肿瘤组织中发现了另外3种酸性变体，其等电点分别为3.2、3.5和3.7。通过体外测量大鼠睾丸间质细胞产生睾酮来确定每种变体的生物活性。与胎盘hCG相对应的等电点4.1部分具有最高的生物活性，而那些远离等电点4.1的部分活性则逐渐降低。所有3种肿瘤特有的酸性变体都具有生物活性，其中聚焦在等电点3.7的部分活性最强。
