Bondy G P, Wilson S, Chambers A F
Cancer Res. 1985 Dec;45(12 Pt 1):6005-9.
We have used a quantitative "experimental" metastasis assay in the embryonic chick, an immunodeficient host, to examine in vivo growth properties of ras oncogene-transformed NIH3T3 cells. We found that two independently derived populations of NIH3T3 cells that had been morphologically transformed with the T24 human H-ras oncogene were able to grow in vivo following i.v. injection. Nontransformed control NIH3T3 cells with normal morphology did not grow in this assay. Spontaneously arising morphological transformants from control NIH3T3 cell populations were also tested and did not grow in this assay. We conclude that the H-ras gene can confer experimental metastatic ability on nonmetastatic NIH3T3 cells, that the ras gene alters the cells in some way beyond in vitro morphological transformation, and thus that the in vitro transformation assay detects only part of the malignant phenotype of these cells.
我们在免疫缺陷宿主胚胎鸡中使用了定量“实验性”转移分析，以研究经ras癌基因转化的NIH3T3细胞的体内生长特性。我们发现，两个独立衍生的、已被T24人H-ras癌基因进行形态转化的NIH3T3细胞群体，经静脉注射后能够在体内生长。形态正常的未转化对照NIH3T3细胞在该分析中不能生长。对来自对照NIH3T3细胞群体自发产生的形态转化体也进行了测试，它们在该分析中同样不能生长。我们得出结论，H-ras基因可赋予非转移性NIH3T3细胞实验性转移能力，ras基因以某种方式改变细胞，超出了体外形态转化的范畴，因此体外转化分析仅检测到这些细胞恶性表型的一部分。
