Allen R G, Newton R K, Farmer K J, Nations C
Cell Tissue Kinet. 1985 Nov;18(6):623-30. doi: 10.1111/j.1365-2184.1985.tb00705.x.
The herbicide paraquat was used to investigate the effects of oxidative stress on the spherulation of Physarum polycephalum microplasmodia. The responses of a white non-differentiating strain of Physarum were compared with those of a common yellow strain that readily spherulates in salts-only starvation medium. The addition of paraquat to the salts medium increased the specific activity of superoxide dismutase in both strains; it also induced an increase in the intracellular inorganic peroxide concentration in both strains. Glutathione concentration was higher in the paraquat-treated yellow strain than in the controls. Paraquat had no effect on glutathione concentration in white microplasmodia. Paraquat accelerated spherulation in yellow microplasmodia. The white microplasmodia responded to the herbicide by cleaving into structures similar to immature spherules; however, these structures were not viable. The results of this study support the hypothesis that free radicals are involved in cell state transitions.
使用除草剂百草枯来研究氧化应激对多头绒泡菌微原质团成球的影响。将多头绒泡菌白色非分化菌株的反应与常见黄色菌株的反应进行比较，后者在仅含盐分的饥饿培养基中很容易形成球体。向盐分培养基中添加百草枯会增加两种菌株中超氧化物歧化酶的比活性；它还会导致两种菌株细胞内无机过氧化物浓度增加。经百草枯处理的黄色菌株中的谷胱甘肽浓度高于对照。百草枯对白色微原质团中的谷胱甘肽浓度没有影响。百草枯加速了黄色微原质团的成球过程。白色微原质团对除草剂的反应是裂解成类似于未成熟球体的结构；然而，这些结构无法存活。本研究结果支持自由基参与细胞状态转变这一假说。
