Liu Yong, Wang Shuo, Zhao Danyang, Zhao Chenglu, Yu Haiqin, Zeng Jianmin, Tong Zhijun, Yuan Cheng, Li Zhenghe, Huang Changjun
Yunnan Academy of Tobacco Agricultural Sciences, Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Kunming, 650021 China.
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058 China.
aBIOTECH. 2025 May 5;6(2):232-248. doi: 10.1007/s42994-025-00216-5. eCollection 2025 Jun.
Recessive resistance mediated by mutations in the eukaryotic translation initiation factor 4E (eIF4E), has proven effective against diverse potyviruses and is extensively utilized in breeding programs. However, the rise of resistance-breaking (RB) strains and emerging potyviral species necessitates the development of more durable and broad-spectrum resistance strategies. In this study, our field survey in Yunnan, China, identified potato virus Y (PVY) RB isolates, as well as the prevalence of tobacco vein banding mosaic virus (TVBMV) and chilli veinal mottle virus (ChiVMV), in tobacco carrying the recessive locus, which lacks the susceptibility gene, due to a chromosomal deletion. Protein interaction and viral infection assays demonstrated that both eIF4E1-S and eIFiso4E-T are used by PVY RB as susceptibility factors for infection, with the combined inactivation of these genes confering durable resistance. Similarly, the knockout of in the genetic background, provided effective resistance to TVBMV and reduced susceptibility to ChiVMV. Notably, pyramiding mutations in and , in tobacco, generated plants exhibiting robust, broad-spectrum resistance, to all three viruses, without compromising plant development. These findings underscore the potential of stacking eIF4E mutations to engineer durable, broad-spectrum resistance to potyviruses in tobacco, offering a promising strategy for crop improvement.
The online version contains supplementary material available at 10.1007/s42994-025-00216-5.
由真核生物翻译起始因子4E（eIF4E）突变介导的隐性抗性已被证明对多种马铃薯Y病毒有效，并在育种计划中广泛应用。然而，抗药性突破（RB）毒株的出现和新出现的马铃薯Y病毒物种使得有必要开发更持久、广谱的抗性策略。在本研究中，我们在中国云南的田间调查发现，携带隐性基因座（由于染色体缺失而缺乏易感基因）的烟草中存在马铃薯Y病毒（PVY）的RB分离株，以及烟草脉带花叶病毒（TVBMV）和辣椒脉斑驳病毒（ChiVMV）的流行情况。蛋白质相互作用和病毒感染试验表明，PVY RB将eIF4E1-S和eIFiso4E-T都用作感染的易感因子，这些基因的联合失活赋予了持久抗性。同样，在该遗传背景下敲除该基因，可有效抵抗TVBMV，并降低对ChiVMV的易感性。值得注意的是，在烟草中对该基因和另一基因进行叠加突变，产生了对所有三种病毒都表现出强大、广谱抗性且不影响植物发育的植株。这些发现强调了叠加eIF4E突变以培育对烟草中马铃薯Y病毒具有持久、广谱抗性的潜力，为作物改良提供了一种有前景的策略。
在线版本包含可在10.1007/s42994-025-00216-5获取的补充材料。
