Zhang Hui, Wang Tao, Gu Jianwei, Hong Dengfeng
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070 China.
College of Life Science and Technology, Hubei Engineering University, Xiaogan, 432000 China.
aBIOTECH. 2025 May 22;6(2):249-262. doi: 10.1007/s42994-025-00217-4. eCollection 2025 Jun.
Varieties with a semi-dwarf compact plant architecture may increase yield per unit area in rapeseed () by allowing high-density cultivation and mechanical harvesting while conferring lodging resistance. Mutation of (), which encodes a receptor-like protein kinase, generates a compact and upright plant architecture in ; however, there have been no reports on the roles of the ER family (ERf) in . In this study, we used the CRISPR/Cas9 system to generate mutants in each of the two homoeologs of members and (), and in the single gene, resulting in the homozygous mutants , /, and . Under greenhouse conditions, / plants were shorter than the wild type, with a compact inflorescence and shorter siliques. In addition, / plants produced significantly more branches and total siliques than the wild type, with no significant changes in the number of ovules per silique or thousand-seed weight. Under field conditions, the mutant plant showed a phenotype comparable to that under greenhouse conditions, but with a notable drop in thousand-seed weight. These results indicate that the mutant offers a valuable germplasm resource for breeding rapeseed with ideal plant architecture.
The online version contains supplementary material available at 10.1007/s42994-025-00217-4.
具有半矮化紧凑株型的品种可能通过允许高密度种植和机械收获同时赋予抗倒伏性来提高油菜籽的单位面积产量。编码类受体蛋白激酶的BnER1突变会在油菜中产生紧凑且直立的株型；然而，关于ER家族（ERf）在油菜中的作用尚无报道。在本研究中，我们使用CRISPR/Cas9系统在BnERf1成员BnERf1.1和BnERf1.2的两个同源基因以及单个BnERf2基因中分别产生突变体，从而得到纯合突变体BnERf1.1/1.1、BnERf1.1/1.2和BnERf2/2。在温室条件下，BnERf1.1/1.2植株比野生型矮，但花序紧凑且角果较短。此外，BnERf1.1/1.2植株产生的分枝和总角果数显著多于野生型，每个角果的胚珠数或千粒重没有显著变化。在田间条件下，BnERf2/2突变体植株表现出与温室条件下相当的表型，但千粒重显著下降。这些结果表明，BnERf2/2突变体为培育具有理想株型的油菜提供了宝贵的种质资源。
在线版本包含可在10.1007/s42994-025-00217-4获取的补充材料。
