Li Ping, Yuan Yongjuan, Yan Yong, Jia Miaomiao, Gao Lei, Liu Xuejuan, Sun Yangming, Zhu Guoying, Chen Zhongwen
Jiaxing Center for Disease Control and Prevention, Jiaxing, China.
Jiashan County Center for Disease Control and Prevention, Jiaxing, China.
Front Microbiol. 2025 Jun 26;16:1620907. doi: 10.3389/fmicb.2025.1620907. eCollection 2025.
is an important cause of foodborne diarrheal diseases worldwide. The emergence of -positive carbapenem-resistant isolates in recent years poses a huge public health challenge.
In this study, two clinical isolates carrying : a serotype 4,[5],12:i:- strain (2023JX045) and a serovar Stanley strain (2024-406) were analyzed using antimicrobial susceptibility testing and whole genome sequencing.
Both isolates were multidrug resistant, with insusceptibility to ampicillin, ampicillin/sulbactam, amoxicillin/clavulanic acid, cefuroxime, ceftiofur, cefazolin, cefoxitin, cefotaxime, ceftazidime, cefepime, ceftazidime/avibactam, meropenem, imipenem, ertapenem, tetracycline, gentamicin, trimethoprim/sulfamethoxazole, florfenicol, chloramphenicol, ciprofloxacin, colistin, and polymixin B. The -carrying plasmids in 2023JX045 and 2024-406 were named p23045-NDM5 and p2024406-NDM5, respectively, with both belonging to the incompatibility (Inc)HI2/IncHI2A group and sequence type ST3. In 2023JX045, was flanked by two same-oriented copies of IS elements (IS- - -IS-ΔIS-ΔIS-IS). In 2024-406, p2024406-NDM5 was found to carry two copies of , possibly resulting from duplication of IS-- - -IS5-ΔISAba125-ΔIS3000-ΔISKox3-umuC-umuD-IS26 and interrupted by mobile element IS at the upstream region of ΔIS3000.
This is the first report to describe the presence of two copies on an IncHI2/IncHI2A plasmid carried by serovar Stanley, as well as the dissemination of in Salmonella in Jiaxing City, China. IS26-flanked composite transposons appeared to play an important role in the formation of this region. The dissemination of blaNDM in Salmonella isolates and the complexity of the region highlight the urgent need to monitor carbapenem-resistant .
是全球食源性腹泻疾病的重要病因。近年来携带 blaNDM 的耐碳青霉烯类分离株的出现带来了巨大的公共卫生挑战。
在本研究中，使用抗菌药物敏感性试验和全基因组测序分析了两株携带 blaNDM 的临床分离株：一株血清型 4,[5],12:i:- 菌株（2023JX045）和一株斯坦利血清型菌株（2024 - 406）。
两株分离株均对多种药物耐药，对氨苄西林、氨苄西林/舒巴坦、阿莫西林/克拉维酸、头孢呋辛、头孢噻呋、头孢唑林、头孢西丁、头孢噻肟、头孢他啶、头孢吡肟、头孢他啶/阿维巴坦、美罗培南、亚胺培南、厄他培南、四环素、庆大霉素、甲氧苄啶/磺胺甲恶唑、氟苯尼考、氯霉素、环丙沙星、黏菌素和多黏菌素 B 不敏感。2023JX045 和 2024 - 406 中携带 blaNDM 的质粒分别命名为 p23045 - NDM5 和 p2024406 - NDM5，二者均属于不相容性（Inc）HI2/IncHI2A 组且序列类型为 ST3。在 2023JX045 中，blaNDM 两侧为两个同向的插入序列拷贝（IS - blaNDM - - - IS - ΔIS - ΔIS - IS）。在 2024 - 406 中，发现 p2024406 - NDM5 携带两个 blaNDM 拷贝，则可能是由 IS - - - - IS5 - ΔISAba125 - ΔIS3000 - ΔISKox3 - umuC - umuD - IS26 的重复导致，并在 ΔIS3000 的上游区域被移动元件 IS 中断。
这是首次报道描述斯坦利血清型携带的 IncHI2/IncHI2A 质粒上存在两个 blaNDM 拷贝，以及 blaNDM 在我国嘉兴市沙门氏菌中的传播情况。IS26 侧翼的复合转座子似乎在该区域的形成中起重要作用。blaNDM 在沙门氏菌分离株中的传播以及 blaNDM 区域的复杂性凸显了监测耐碳青霉烯类沙门氏菌的迫切需求。
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