Zhao Kaiting, Jin Jing, Liao Yuan, Liu Aixia, Liu Wugao, Wu Weiping
Department of Hematology, Lishui People's Hospital, The Sixth Affiliated Hospital of Wenzhou Medical University, Lishui, Zhejiang, China.
Department of Clinical Laboratory, Lishui People's Hospital, The Sixth Affiliated Hospital of Wenzhou Medical University, Lishui, Zhejiang, China.
iScience. 2024 Dec 31;28(2):111720. doi: 10.1016/j.isci.2024.111720. eCollection 2025 Feb 21.
Multidrug-resistant Typhimurium has emerged as a global public health concern. Asymptomatic gastrointestinal carriage is a key factor in the spread of antibiotic-resistant bacteria. However, it is challenging to obtain direct evidence of transfer of mobile genetic elements (MGEs). Here, we found that MGEs harboring were transferred from asymptomatic (LS20223694) to Typhimurium (LS20223695) in a child. BLAST analyses demonstrated that the IS-flanked composite transposon of pLS20223695_NDM5 showed high similarity with pLS20223694_NDM5 and two previously reported plasmids, suggesting the possibility of genetic recombination. Besides, conjugation experiments showed that the transconjugant carried the incompatibility group I1 (IncI1)-I(alpha) plasmid replicon with the and genes, indicating that pLS20223695_NDM5 is a conjugative plasmid with transferability. Our study provides insights into the genetic basis of an IS-flanked composite transposon in Typhimurium.
多重耐药鼠伤寒沙门氏菌已成为全球公共卫生关注的问题。无症状胃肠道携带是抗生素耐药菌传播的一个关键因素。然而，获得移动遗传元件（MGEs）转移的直接证据具有挑战性。在此，我们发现携带MGEs的菌株在一名儿童中从无症状的[菌株名称LS20223694]转移到了鼠伤寒沙门氏菌[菌株名称LS20223695]。BLAST分析表明，pLS20223695_NDM5的插入序列侧翼复合转座子与pLS20223694_NDM5以及两个先前报道的质粒具有高度相似性，提示了基因重组的可能性。此外，接合实验表明，接合子携带了不相容群I1（IncI1）-I(α)质粒复制子以及[相关基因名称]基因，表明pLS20223695_NDM5是一种具有转移性的接合性质粒。我们的研究为鼠伤寒沙门氏菌中插入序列侧翼复合转座子的遗传基础提供了见解。
