First Report of in Galapagos: High Prevalence in Dogs and Circumstantial Evidence for the Role of as Vector.

The current study investigates the presence and prevalence of species in dogs from the Galapagos Islands, focusing on the potential vectorial role of in the transmission of these pathogens. Blood samples were collected from 1221 dogs across four islands, with tick collections for morphological and genetic identification. The results revealed a significant molecular prevalence of (20.3%), predominantly in Santa Cruz (35.16%) and Isabela (18.9%), while was identified in 2.9% of samples. Genetic analysis identified the presence of ecotype I, aligning more closely with European strains. Furthermore, was confirmed as the only tick species associated with dogs, suggesting its role as a vector for both and . This study marks the first molecular confirmation of these pathogens in the Galapagos, contributing with important insights into the epidemiology of tick-borne diseases in this ecosystem. The findings highlight the need for improved surveillance and control to reduce the risk and further spread of these tick-borne diseases.