Eggers Ernst-Jan, Su Ying, van Heusden Sjaak A W, de Vries Michiel E, Bachem Christian W B, Visser Richard G F, Lindhout Pim
Solynta, Dreijenlaan 2, 6703 HA Wageningen, The Netherlands.
Graduate School Experimental Plant Sciences, Wageningen University & Research, Wageningen, The Netherlands.
Potato Res. 2025;68(2):1459-1476. doi: 10.1007/s11540-024-09792-3. Epub 2024 Sep 13.
Genetic gain in potato breeding is limited by the heterozygous tetraploid genome of cultivated potato. Recent efforts to breed potato at the diploid level promise to improve genetic gain and allow more straightforward genetics and introgression breeding. Diploid F1 hybrid potato breeding relies on the ability to create diploid inbred lines via repeated self-fertilization. However, self-fertilization of diploid potato is hampered by a gametophytic self-incompatibility system encoded by the S-locus that prevents fertilization by self-pollen. Nonetheless, self-compatible diploid potato genotypes exist and have been used to create inbred lines. The () gene is a dominant gene that provides strong self-compatibility in diploid potato and was previously mapped to Chromosome 12. While the gene has already been identified and characterized, the most tedious challenge was to develop the optimal phenotyping methods and genetic populations preceding the cloning of this gene. To this end, we developed an effective phenotyping protocol to identify suitable parents and create diploid populations segregating for . We show that an accurate phenotyping method is crucial to discriminate between confounding fertility factors and self-compatibility. In addition, we found that the locus shows extreme segregation distortion on Chromosome 12. Finally, we used these insights to develop three F1 populations that segregate for , which we later used for the identification of the gene.
The online version contains supplementary material available at 10.1007/s11540-024-09792-3.
栽培马铃薯的遗传增益受到其杂合四倍体基因组的限制。近期在二倍体水平上进行马铃薯育种的努力有望提高遗传增益，并使遗传学和渐渗育种更加直接。二倍体F1杂交马铃薯育种依赖于通过反复自交来创建二倍体自交系的能力。然而，二倍体马铃薯的自交受到由S位点编码的配子体自交不亲和系统的阻碍，该系统可防止自花花粉受精。尽管如此，自交亲和的二倍体马铃薯基因型是存在的，并已被用于创建自交系。（）基因是一个显性基因，可在二倍体马铃薯中提供强大的自交亲和性，此前已被定位到12号染色体上。虽然该基因已被鉴定和表征，但最繁琐的挑战是在克隆该基因之前开发出最佳的表型分析方法和遗传群体。为此，我们开发了一种有效的表型分析方案，以识别合适的亲本并创建用于（）基因分离的二倍体群体。我们表明，准确的表型分析方法对于区分混杂的育性因素和自交亲和性至关重要。此外，我们发现（）基因座在12号染色体上表现出极端的分离畸变。最后，我们利用这些见解开发了三个用于（）基因分离的F1群体，随后我们将其用于该基因的鉴定。
在线版本包含可在10.1007/s11540-024-09792-3获取的补充材料。
