Shen Chen, Zheng Lu, Li Lingling, Li Mingzhe, Zhang Yantian, Guo Qiangzhi, Yu Anfeng, Wang Yuejie
State Key Laboratory of Chemical Safety, SINOPEC Research Institute of Safety Engineering Co., Ltd, Qingdao 266100, Shandong, PR China.
SINOPEC National Petrochemical Project Risk Assessment Technical Center Co. Ltd, PR China.
Environ Sci Process Impacts. 2025 Aug 13;27(8):2519-2527. doi: 10.1039/d5em00400d.
Oilfield-produced water, a byproduct of oil and gas extraction, contains organic contaminants and inorganic metals that can pose a risk to the human health and environmental safety. Luminescent bacteria are frequently utilized as bioassay species in toxicity assessments, particularly in the context of wastewater, water streams, contaminated sites, and chemical substances. Given the disadvantages associated with the reliance of marine luminescent bacteria on high salinity, herein, the recombinant strains were employed as the bioassay species for toxicity assessment. In this study, a widespread sodium chloride (NaCl)-tolerant, pH-adaptable and steady-luminescent sensor, DH5a (pGEN), was recombined. This strain exhibited a comparatively higher sensitivity to the toxicity of oilfield-produced water than the typical toxicity test species 502. In contrast to freshwater samples, the recombined luminescent sensor was a better choice to determine the toxicity of salinity-sensitive wastewater samples. In addition, depending on the chemical compositions and physicochemical parameters of the PW samples, the primary intoxicants were analyzed by correlating the toxicity values with the concentrations of arsenic and boron. The findings underscored the imperative for the employment of suitable bioassays to optimize the categorization of the produced water, with a view to reduce leakage and associated environmental hazards.
油田采出水是油气开采的副产品，含有有机污染物和无机金属，会对人类健康和环境安全构成风险。发光细菌经常被用作毒性评估中的生物测定物种，特别是在废水、水流、污染场地和化学物质的背景下。鉴于海洋发光细菌依赖高盐度存在的缺点，本文采用重组菌株作为毒性评估的生物测定物种。在本研究中，构建了一种广泛存在的耐氯化钠（NaCl）、适应pH且发光稳定的传感器DH5a（pGEN）。该菌株对油田采出水毒性的敏感性比典型毒性测试物种502更高。与淡水样品相比，重组发光传感器是测定对盐度敏感的废水样品毒性的更好选择。此外，根据采出水（PW）样品的化学成分和理化参数，通过将毒性值与砷和硼的浓度相关联来分析主要毒物。研究结果强调了采用合适的生物测定方法来优化采出水分类的必要性，以减少泄漏及相关环境危害。
