Semmate Noha, Bamouh Zahra, Elkarhat Zouhair, Bkiri Dounia, Elmejdoub Soufiane, Kerbal Ismail, Tadlaoui Khalid Omari, Elharrak Mehdi
Department of Research and Development, Multi-Chemical Industry Santé Animale, Mohammadia 28810, Morocco,
Department of Pathology and Veterinary Public Health, Agronomic and Veterinary Institute Hassan II, B.P. 6202, Rabat-Instituts, Rabat, Morocco.
Avian Dis. 2025 Jun;69(2):200-205. doi: 10.1637/aviandiseases-D-24-00074.
is an important pathogen that causes fowl cholera (FC) in turkeys (). The aim of this study was to isolate and characterize from outbreaks of FC in turkeys for the first time in Morocco, both molecularly and pathologically, and to develop and evaluate an inactivated vaccine based on the isolated strain. Two farms in central Morocco were suspected of having FC. From each of these farms, two isolates were characterized. Molecular analysis showed that both strains were identified as genotype A and serovars 3*4. Three oil-based vaccines were prepared using the virulent isolate strain after evaluating its pathogenicity in target species to determine the protective dose. The vaccines were evaluated through challenge tests and serologic responses. According to the results, a dose of 10 colony-forming units showed 100% protection after challenge, and all animals became seropositive. Additionally, two vaccination programs were proposed to evaluate the duration of immunity: a double vaccination schedule a triple vaccination schedule. The results indicated that the triple vaccination induced good protection after challenge and a high level of antibodies.
是一种重要的病原体，可导致火鸡发生禽霍乱（FC）（）。本研究的目的是首次在摩洛哥从火鸡FC疫情中分离并在分子和病理学上鉴定，以及开发和评估基于分离菌株的灭活疫苗。摩洛哥中部的两个养殖场疑似发生FC。从每个养殖场中鉴定出两个分离株。分子分析表明，这两个菌株均被鉴定为基因型A和血清型3*4。在评估其在目标物种中的致病性以确定保护剂量后，使用强毒株制备了三种油佐剂疫苗。通过攻毒试验和血清学反应对疫苗进行评估。根据结果，10个菌落形成单位的剂量在攻毒后显示出100%的保护作用，所有动物均呈血清阳性。此外，还提出了两种疫苗接种方案来评估免疫持续时间：双次接种方案和三次接种方案。结果表明，三次接种在攻毒后诱导了良好的保护作用和高水平的抗体。
