血小板ADP受体变体rs1371097与印度患者血小板对阿司匹林反应不足的关联。

Association of platelet ADP receptor variant rs1371097 with inadequate platelet response to aspirin in Indian patients.

作者信息

Sundaravadivel Pandarisamy, Christopher Rita, Chandra Sadanandavalli Retnaswami, Ramakrishnan Subasree

机构信息

Department of Neurochemistry, National Institute of Mental Health and Neuro Sciences, Bengaluru, India.

Integrative Medical Research, PES University Institute of Medical Sciences and Research, Bengaluru, India.

出版信息

Per Med. 2025 Jul 11:1-10. doi: 10.1080/17410541.2025.2530381.

DOI:10.1080/17410541.2025.2530381

PMID:40644802

Abstract

AIM

On-aspirin platelet reactivity, wherein patients show sub-optimal or no response to antiplatelet therapy, occurs in 5-60% of subjects. The genetic etiology of such reactivity in patients with vascular diseases, especially ischemic stroke in the Indian population, is unknown. This study aimed to examine the genetic variations in the pathways of platelet aggregation and aspirin metabolism that could predict aspirin response.

METHODS

This is a prospective cohort study, which included 293 ischemic stroke patients on 150 mg aspirin for over 7 days. Platelet aggregation was assessed using light transmission aggregometry with 10 µM ADP and 0.5 mM arachidonic acid as agonist. After excluding patients with serum salicylic acid levels < 30 µg/mL, 230 individuals were analyzed. Candidate gene variants in , and , were genotyped using PCR-RFLP or allelic discrimination assays.

RESULTS

The T allele of (rs1371097 C > T) was significantly associated with inadequate platelet response with an odds ratio of 1.71 (95% CI: 1.122-2.61; = 0.0131). Carriers of this allele had a 3.46-fold increased risk of inadequate response after adjustment for age and gender.

CONCLUSIONS

The (rs1371097 C > T) variant may be a potential genetic marker for inadequate response to aspirin in Indian ischemic stroke patients.

摘要

目的

阿司匹林治疗下的血小板反应性欠佳，即患者对抗血小板治疗显示出次优反应或无反应，在5%至60%的受试者中出现。血管疾病患者，尤其是印度人群中的缺血性中风患者，这种反应性的遗传病因尚不清楚。本研究旨在检查血小板聚集和阿司匹林代谢途径中的基因变异，这些变异可预测阿司匹林反应。

方法

这是一项前瞻性队列研究，纳入了293名服用150毫克阿司匹林超过7天的缺血性中风患者。使用光透射聚集法，以10微摩尔/升二磷酸腺苷（ADP）和0.5毫摩尔/升花生四烯酸作为激动剂评估血小板聚集。排除血清水杨酸水平<30微克/毫升的患者后，对230名个体进行分析。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）或等位基因鉴别分析对、和中的候选基因变异进行基因分型。