Guardamagna Matteo, Chadney Oscar, Stella Federico, Zhang Qiangwei, Kentros Clifford, Battaglia Francesco P
Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, Trondheim, Norway.
Donders Institute for Brain, Cognition and Behaviour, Radboud University, Nijmegen, the Netherlands.
Nat Commun. 2025 Jul 11;16(1):6430. doi: 10.1038/s41467-025-61453-2.
Theta sequences of hippocampal activity supports planning and prediction, and in CA1 they are shaped by CA3 and entorhinal (layer III) inputs. We targeted entorhinal inputs with highly specific optogenetic inhibition, leaving the remaining circuit intact. While CA1 spatial coding properties were largely unaffected, the slope and range of theta phase precession were impaired. Surprisingly, theta sequences were strengthened. These results suggest that sequence organization is not a simple consequence of precession and depends on circuit-level dynamics across the trisynaptic circuit, while direct entorhinal inputs may act as a supervisory signal driving learning and representational updates.
海马体活动的θ序列支持计划和预测，在CA1区，它们由CA3区和内嗅皮层（III层）的输入塑造。我们通过高度特异性的光遗传学抑制作用靶向内嗅皮层的输入，而其余回路保持完整。虽然CA1区的空间编码特性基本未受影响，但θ相位进动的斜率和范围受损。令人惊讶的是，θ序列得到了增强。这些结果表明，序列组织不是进动的简单结果，而是取决于三突触回路的回路水平动力学，而直接的内嗅皮层输入可能作为驱动学习和表征更新的监督信号。
