Yu Aiying, Wang Zhenjiang, Wang Sutian, Zhao Weiguo, Chen Lian, Wang Dan, Li Zhiyi, Wang Yuan, Fang Zhengfeng, Lin Sen
Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China.
Key Laboratory of Urban Agriculture in South China, Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Animals (Basel). 2025 Jun 20;15(13):1826. doi: 10.3390/ani15131826.
This study aims to investigate the effects of dietary proanthocyanidins (PACs) on growth performance, intestinal inflammation and barrier function, and bile acid metabolism-related genes in weaned piglets challenged with lipopolysaccharide (LPS). A total of 18 21-day-old castrated piglets (7.16 ± 1.66 kg) were randomly assigned to three groups: (1) CON (a basal diet), (2) LPS (a basal diet + LPS), (3) LPS + PAC (a basal diet + LPS + 250 mg/kg PAC), with each group consisting of six replicates of 1 piglet per treatment. The study lasted for 21 days. On the 14th and 21st days of the experiment, piglets in the LPS and LPS + PAC groups received an intraperitoneal injection of 100 µg/kg body weight of LPS, while the piglets in the CON group received an injection of 0.9% normal saline solution. The LPS + PAC group exhibited a significantly higher average daily gain (ADG) than the LPS group ( < 0.05). LPS stimulation resulted in a decreased ( < 0.05) villus height of the jejunum and ileum and an increased number of goblet cells. These effects were alleviated ( < 0.05) in the LPS + PAC group. The LPS + PAC group decreased the level of TNF-α and D-lactate in serum and the gene expression of and in the ileal tissue, compared with the LPS group, while increasing the gene expression of and in the ileal tissue ( < 0.05). LPS stimulation down-regulated the expression of genes regulating bile acid synthesis and transport, including hepatic and ileum , whereas dietary PAC had no significant effect on the expression of these genes ( > 0.05). Nevertheless, supplementation with PAC significantly increased the expression levels of , , and in the ileum of piglets ( < 0.05). Additionally, piglets in the LPS + PAC group exhibited a significant increase in the level of glucagon-like peptide 2 (GLP-2) compared with the LPS group ( < 0.05). PAC generally improves the ADG, intestinal morphology, and intestinal barrier function of piglets by activating TGR5 to stimulate the intestinal secretion of GLP-2.
本研究旨在探讨日粮原花青素（PACs）对脂多糖（LPS）攻毒断奶仔猪生长性能、肠道炎症和屏障功能以及胆汁酸代谢相关基因的影响。选取18头21日龄去势仔猪（体重7.16±1.66 kg），随机分为3组：（1）CON组（基础日粮）；（2）LPS组（基础日粮+LPS）；（3）LPS+PAC组（基础日粮+LPS+250 mg/kg PAC），每组6个重复，每个重复1头仔猪。试验持续21天。在试验的第14天和第21天，LPS组和LPS+PAC组仔猪腹腔注射100 μg/kg体重的LPS，CON组仔猪注射0.9%生理盐水。LPS+PAC组的平均日增重（ADG）显著高于LPS组（P<0.05）。LPS刺激导致空肠和回肠绒毛高度降低（P<0.05），杯状细胞数量增加。LPS+PAC组这些影响得到缓解（P<0.05）。与LPS组相比，LPS+PAC组降低了血清中TNF-α和D-乳酸水平以及回肠组织中TLR4和MyD88的基因表达，同时增加了回肠组织中TGR5和FXR的基因表达（P<0.05）。LPS刺激下调了调节胆汁酸合成和转运的基因表达，包括肝脏CYP7A1和回肠FGF15，而日粮PAC对这些基因的表达无显著影响（P>0.05）。然而，补充PAC显著增加了仔猪回肠中TGR5、FXR和GLP-2的表达水平（P<0.05）。此外，与LPS组相比，LPS+PAC组仔猪胰高血糖素样肽2（GLP-2）水平显著升高（P<0.05）。PAC通常通过激活TGR5刺激肠道分泌GLP-2来改善仔猪的ADG、肠道形态和肠道屏障功能。
