Li Liping, Hu Dayan, Yu Jiongying, Zheng Xingyu, Cai Miaoying, Gao Quanxin, Yi Shaokui
School of Life Sciences, Huzhou University, Huzhou 313000, China.
Huzhou Academy of Agricultural Sciences, Huzhou 313000, China.
Animals (Basel). 2025 Jun 29;15(13):1917. doi: 10.3390/ani15131917.
The giant freshwater prawn (, GFP) is a highly valuable crustacean species in global aquaculture. However, a social hierarchy exists among the distinct male morphotypes, specifically blue-clawed males (BC), orange-clawed males (OC), and small males (SMs). In this study, to identify the specific metabolites among BC, OC, and SM, hemolymph samples were collected for the untargeted liquid chromatography-mass spectrometry metabolomics (LC-MS). A total of 172, 546, and 578 significantly different metabolites (SDMs) were identified in OC vs. BC, SM vs. BC, and SM vs. OC, respectively. Notably, creatine and glutamate in BC males likely enhance their aggressive behavior through improved energy metabolism. In the SM group, the up-regulation of prostaglandin E3, testosterone, and arachidonic acid may lead to premature gonadal maturation and enhance immunity. Serotonin, Glu-Pro, and pentanoylcarnitine detected in OC males reflect their physiological need for rapid growth and adaptation to social behaviors. In the SM group, the up-regulation of prostaglandin E3, arachidonic acid, and testosterone may promote premature gonadal maturation and enhance immunodominance. These findings will enhance the understanding of the physiological basis of social hierarchy formation in male GFPs from a metabolomics perspective.
罗氏沼虾（GFP）是全球水产养殖中一种极具价值的甲壳类物种。然而，在不同的雄性形态类型之间存在社会等级制度，具体为蓝爪雄虾（BC）、橙爪雄虾（OC）和小雄虾（SM）。在本研究中，为了鉴定BC、OC和SM之间的特定代谢物，采集了血淋巴样本用于非靶向液相色谱 - 质谱代谢组学（LC - MS）分析。在OC与BC、SM与BC以及SM与OC的比较中，分别鉴定出172、546和578种显著差异代谢物（SDM）。值得注意的是，BC雄虾中的肌酸和谷氨酸可能通过改善能量代谢来增强其攻击行为。在SM组中，前列腺素E3、睾酮和花生四烯酸的上调可能导致性腺过早成熟并增强免疫力。在OC雄虾中检测到的血清素、Glu - Pro和戊酰肉碱反映了它们对快速生长和适应社会行为的生理需求。在SM组中，前列腺素E3、花生四烯酸和睾酮的上调可能促进性腺过早成熟并增强免疫优势。这些发现将从代谢组学角度增强对雄性罗氏沼虾社会等级制度形成的生理基础的理解。
