Hu Jiahuan, Zhao Wei, Zhao Jinyan, Tian Jinli, Yang Lijuan, Wang Hua, Chen Siyu, Ma Ruimin, Gu Yaling, Wei Dawei, Zhang Juan
College of Animal Science and Technology, Ningxia University, Yinchuan 750000, China.
Animals (Basel). 2025 Jul 1;15(13):1938. doi: 10.3390/ani15131938.
In this study, we investigated the dynamic changes in muscle lipid content (MLC) deposition in the breast muscle of Jingyuan chickens at different stages (42-, 126-, and 180- days old) using metabolomics. The pH a*, and L* were significantly increased in the breast muscle of 126-day-old chickens, and MLC and b* were significantly increased in the breast muscle of 180-day-old chickens. A total of 4643 differentially expressed metabolites (DEMs) were identified, of which 10 decreased and 29 increased with age. Key pathways associated with meat quality traits included oxidative phosphorylation, β-alanine metabolism, and glycerophospholipid metabolism. Combined transcriptomic and phenotypic correlation analyses revealed significant positive correlations of MLC, pH, a*, and L* with LysoPC 20:4, , , , , and . In the 42- and 180-day-old chickens, MLC, pH, a*, and L* were significantly and positively correlated with L-Anserine, Dihydroxyacetone phosphate, , and . MLC, pH, a*, and L* were significantly and positively correlated with beta-Nicotinamide adenine dinucleotide in the 126- and 180-day-old chickens. This study deepens our understanding of the differences in MLC deposition at different stages of Jingyuan chickens and its relationship with meat quality traits.
在本研究中，我们运用代谢组学方法，调查了不同阶段（42日龄、126日龄和180日龄）静原鸡胸肌中肌肉脂质含量（MLC）沉积的动态变化。126日龄鸡的胸肌中pH值、a值和L值显著升高，180日龄鸡的胸肌中MLC和b值显著升高。共鉴定出4643种差异表达代谢物（DEM），其中10种随年龄增长而减少，29种随年龄增长而增加。与肉质性状相关的关键途径包括氧化磷酸化、β-丙氨酸代谢和甘油磷脂代谢。转录组和表型相关性联合分析显示，MLC、pH值、a值和L值与溶血磷脂酰胆碱20:4等显著正相关。在42日龄和180日龄的鸡中，MLC、pH值、a值和L值与L-鹅肌肽、磷酸二羟丙酮等显著正相关。在126日龄和180日龄的鸡中，MLC、pH值、a值和L*值与β-烟酰胺腺嘌呤二核苷酸显著正相关。本研究加深了我们对静原鸡不同阶段MLC沉积差异及其与肉质性状关系的理解。
