Ningxia Key Laboratory of Ruminant Molecular and Cellular Breeding, College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China.
Ningxia Key Laboratory of Ruminant Molecular and Cellular Breeding, College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China.
Food Chem. 2025 Feb 1;464(Pt 2):141732. doi: 10.1016/j.foodchem.2024.141732. Epub 2024 Oct 22.
Local chicken breeds in China are highly regarded for their superior meat flavor. This study utilized lipidomics and non-targeted metabolomics to identify biomarkers influencing intramuscular fat (IMF) deposition in the breast muscle of 42- and 180-day-old Jingyuan chickens. Results revealed that IMF content was higher in the breast muscle of 180-day-old Jingyuan chickens compared to 42-day-old chickens (P < 0.01). We identified 248 differentially expressed lipids (DELs) and 1042 differentially expressed metabolites (DEMs). The breast muscle of 180-day-old chickens contained higher levels of TG, fatty acid (FA) and cholesteryl ester (CE), with C16:1 and C18:1 being particularly abundant. Integration of non-targeted metabolomic analyses emphasized glycerolipid metabolism and vitamin digestion and absorption as the main pathways distinguishing between 42- and 180-day-old chickens. Additionally, the differential metabolites LysoPS 18:1, LysoPC 20:3, LysoPC 18:2, LysoPI 20:3, and Pantothenic acid contributed to enhanced meat flavor in Jingyuan chickens.
中国的地方鸡种以其卓越的肉质风味而备受推崇。本研究利用脂质组学和非靶向代谢组学技术，鉴定了影响景园鸡 42 日龄和 180 日龄胸肌肌内脂肪（IMF）沉积的生物标志物。结果表明，180 日龄景园鸡的胸肌 IMF 含量高于 42 日龄（P<0.01）。我们共鉴定出 248 种差异表达脂质（DELs）和 1042 种差异表达代谢物（DEMs）。180 日龄鸡的胸肌中 TG、FA 和 CE 含量较高，其中 C16:1 和 C18:1 特别丰富。非靶向代谢组学分析的整合强调甘油磷脂代谢和维生素消化吸收是区分 42 日龄和 180 日龄鸡的主要途径。此外，差异代谢物 LysoPS 18:1、LysoPC 20:3、LysoPC 18:2、LysoPI 20:3 和泛酸有助于提高景园鸡的肉味。
