Yu Shui-Sheng, Xiang Jing-Wen, Zhang Lin, Guo Xiao-Hua, Wang Yu, Ding Guo-Hua, Hu Hua-Li
Laboratory of Amphibian Diversity Investigation, College of Ecology, Lishui University, Lishui 323000, China.
Administration Center of Zhejiang Jiulongshan National Nature Reserve, Suichang, Lishui 323312, China.
Animals (Basel). 2025 Jul 4;15(13):1973. doi: 10.3390/ani15131973.
The intestinal microbiota regulates host metabolic homeostasis through production of bioactive microbial metabolites. These microorganisms facilitate digestion, enhance immune function, maintain osmoregulation, and support physiological balance via these bioactive compounds, thereby enhancing environmental adaptation. Our study investigated intestinal microbiota-liver metabolic interactions in using 16S rRNA gene sequencing and non-targeted liquid chromatography-tandem mass spectrometry metabolomics. Key findings include (1) comparable alpha diversity but distinct microbial community structures between the small intestine (SI) and large intestine (LI), with the SI dominated by Enterobacteriaceae (72.14%) and the LI by Chitinophagaceae (55.16%); (2) segment-specific microbe-metabolite correlations, with predominantly positive correlations in the SI and complex patterns in the LI involving fatty acids, amino acids, and energy metabolites; and (3) significant correlations between specific bacterial families (Aeromonadaceae, Enterobacteriaceae, Chitinophagaceae) and hepatic metabolites related to fatty acid metabolism, amino acid synthesis, and energy pathways, indicating potential gut-liver axis associations. These findings provide insights into amphibian intestinal microbiota-hepatic metabolite associations and may inform future studies of host-microbe interactions.
肠道微生物群通过产生生物活性微生物代谢物来调节宿主代谢稳态。这些微生物通过这些生物活性化合物促进消化、增强免疫功能、维持渗透压调节并支持生理平衡，从而增强环境适应性。我们的研究使用16S rRNA基因测序和非靶向液相色谱-串联质谱代谢组学研究了[未提及具体对象]中的肠道微生物群-肝脏代谢相互作用。主要发现包括：（1）小肠（SI）和大肠（LI）之间的α多样性相当，但微生物群落结构不同，SI中以肠杆菌科为主（72.14%），LI中以噬几丁质菌科为主（55.16%）；（2）特定节段的微生物-代谢物相关性，SI中主要为正相关，LI中为涉及脂肪酸、氨基酸和能量代谢物的复杂模式；（3）特定细菌科（气单胞菌科、肠杆菌科、噬几丁质菌科）与肝脏中与脂肪酸代谢、氨基酸合成和能量途径相关的代谢物之间存在显著相关性，表明潜在的肠-肝轴关联。这些发现为两栖动物肠道微生物群-肝脏代谢物关联提供了见解，并可能为未来宿主-微生物相互作用的研究提供参考。
