Zhang Kai, Yan Shuhui, Miao Junhong, Li Wen, Li Zhenxu
College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
Animals (Basel). 2025 Jul 7;15(13):1989. doi: 10.3390/ani15131989.
This study investigated the protective effects of high selenomethionine (SeMet) supplementation on liver injury caused by oxidative stress in piglets and explored the underlying mechanisms. A total of 18 piglets were randomly assigned to three groups, with six replicates in each group. The control (CON) and diquat (DQ) groups were fed a basal diet supplemented with 0.3 mg Se/kg Se, while the SeMet group received a basal diet supplemented with 1.0 mg Se/kg. The results indicated that SeMet supplementation significantly improved growth performance and increased the serum and liver activities of antioxidant enzymes. Additionally, it reduced the serum and liver levels of malondialdehyde and protein carbonyls in piglets exposed to DQ. Selenoprotein transcriptome analysis showed that the mRNA levels of five selenoprotein genes (, , and ) were significantly upregulated by dietary SeMet supplementation in the liver of DQ-challenged piglets. Proteomic analysis revealed that a total of 3614 proteins were identified in the liver of piglets. Among them, 85 differentially expressed proteins (DEPs) were identified between the CON and DQ groups, 58 DEPs were observed between the DQ and SeMet groups, and 113 DEPs were identified between the CON and SeMet groups. KEGG analysis indicated that most of the DEPs observed among the three groups were involved in fatty acid metabolism, glycolysis/gluconeogenesis, and the PPAR signaling pathway. Together, these results indicate that dietary supplementation with supernutritional SeMet alleviates the negative effects of the DQ challenge on growth performance and liver injury in piglets. This effect is associated with increased antioxidant capacity, enhanced expression of certain selenoprotein genes, and the regulation of fatty acid metabolism.
本研究调查了高剂量硒代蛋氨酸（SeMet）补充剂对仔猪氧化应激所致肝损伤的保护作用，并探究其潜在机制。总共18头仔猪被随机分为三组，每组六个重复。对照组（CON）和百草枯（DQ）组饲喂添加0.3 mg Se/kg硒的基础日粮，而SeMet组接受添加1.0 mg Se/kg硒的基础日粮。结果表明，补充SeMet显著改善了生长性能，并提高了抗氧化酶的血清和肝脏活性。此外，它降低了暴露于DQ的仔猪血清和肝脏中的丙二醛和蛋白质羰基水平。硒蛋白转录组分析表明，日粮补充SeMet显著上调了DQ攻击仔猪肝脏中五个硒蛋白基因（、和）的mRNA水平。蛋白质组学分析显示，在仔猪肝脏中总共鉴定出3614种蛋白质。其中，CON组和DQ组之间鉴定出85种差异表达蛋白（DEPs），DQ组和SeMet组之间观察到58种DEPs，CON组和SeMet组之间鉴定出113种DEPs。KEGG分析表明，三组中观察到的大多数DEPs参与脂肪酸代谢、糖酵解/糖异生和PPAR信号通路。总之，这些结果表明，超营养剂量的SeMet日粮补充可减轻DQ攻击对仔猪生长性能和肝损伤的负面影响。这种作用与抗氧化能力增强、某些硒蛋白基因表达增加以及脂肪酸代谢调节有关。
