The Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, China.
The Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, China.
Food Chem Toxicol. 2022 Dec;170:113480. doi: 10.1016/j.fct.2022.113480. Epub 2022 Oct 17.
Selenium nanoparticles (SeNPs) with unique biological properties have been suggested as a safer and more effective platform for delivering of Selenium for biological needs. In this study, we investigated the association between gut microbiota altered by SeNPs supplementation and its metabolites under oxidative stress conditions through 16S rDNA gene sequencing analysis and untargeted metabolomics. The results showed that dietary supplementation with SeNPs attenuated diquat-induced acute toxicity in mice, as demonstrated by lower levels of inflammatory effector cells, and biochemical markers in serum such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN) and lactate dehydrogenase (LDH). SeNPs also reversed the perturbed gut microbiota composition induced by diquat, decreased the Firmicutes/Bacteroidetes ratio, and increased the abundance of beneficial bacteria such as Akkermansia, Muribaculaceae, Bacteroides and Parabacteroides. Untargeted fecal metabolomics showed that SeNPs can regulate the production of steroids and steroid derivatives, organonitrogen compounds, pyridines and derivatives and other metabolites. Microbiome-metabolome correlation analysis suggested that Parabacteroides was the key bacteria for the SeNPs intervention, which might upregulate the levels of metabolites such as trimethaphan, emedastine, berberine, desoxycortone, tetrahydrocortisone. This study demonstrated that dietary SeNPs supplementation can extensively modulate the gut microbiota and its metabolism, thereby alleviating diquat-induced acute toxicity.
硒纳米颗粒（SeNPs）具有独特的生物学特性，被认为是一种更安全、更有效的输送硒的平台，以满足生物需求。在这项研究中，我们通过 16S rDNA 基因测序分析和非靶向代谢组学研究了 SeNPs 补充改变的肠道微生物群及其在氧化应激条件下的代谢物之间的关联。结果表明，膳食补充 SeNPs 可减轻百草枯诱导的小鼠急性毒性，表现为炎症效应细胞和血清生化标志物如天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、血尿素氮（BUN）和乳酸脱氢酶（LDH）水平降低。SeNPs 还逆转了百草枯引起的肠道微生物群组成的紊乱，降低了厚壁菌门/拟杆菌门的比例，增加了有益细菌如阿克曼氏菌、Muribaculaceae、拟杆菌和副拟杆菌的丰度。非靶向粪便代谢组学显示，SeNPs 可以调节类固醇和类固醇衍生物、有机氮化合物、吡啶及其衍生物和其他代谢物的产生。微生物组-代谢组关联分析表明，副拟杆菌是 SeNPs 干预的关键细菌，它可能上调三甲氧苯嗪、乙替丁、小檗碱、脱氧皮质酮、四氢皮质酮等代谢物的水平。本研究表明，膳食 SeNPs 补充可以广泛调节肠道微生物群及其代谢，从而减轻百草枯诱导的急性毒性。
