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金属-酚醛网络导向涂层:一种提高稳定性的有前景策略。

Metal-Phenolic Network-Directed Coating of : A Promising Strategy to Increase Stability.

作者信息

Zhang Haoxuan, Zhang Huange, Zhong Hao

机构信息

College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China.

出版信息

Foods. 2025 Jun 26;14(13):2277. doi: 10.3390/foods14132277.


DOI:10.3390/foods14132277
PMID:40647029
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12248584/
Abstract

exhibits probiotic effects, including regulating the balance of the intestinal microbiota and enhancing immune function. However, this strain often experiences viability loss upon ingestion due to harsh conditions within the human digestive tract. This study aimed to evaluate the efficacy of metal-phenol networks (MPNs) fabricated via three polyphenols-tannic acid (TA), tea polyphenol (TP), and anthocyanin (ACN)-combined with Fe(III) coatings in protecting during simulated digestion and storage. The results demonstrated that MPNs formed a protective film on the bacterial surface. While TA and ACN inhibited the growth of YJ7, TP stimulated proliferation. Within the MPNs system, only Fe(III)-TA exhibited growth-inhibitory effects. Notably, ACN displayed the highest proliferation rate during the initial 2 h, followed by TP between 3 and 4 h. All MPN-coated groups maintained high bacterial viability at 25 °C and -20 °C, with TP-coated bacteria showing the highest viable cell count, followed by TA and ACN. In vitro digestion experiments further revealed that the Fe(III)-ACN group exhibited the strongest resistance to artificial gastric juice. In conclusion, tea polyphenol and anthocyanin demonstrate superior potential for probiotic encapsulation, offering both protective stability during digestion and enhanced viability under storage conditions.

摘要

具有益生菌作用,包括调节肠道微生物群平衡和增强免疫功能。然而,由于人类消化道内的恶劣条件,这种菌株在摄入后常常会出现活力丧失的情况。本研究旨在评估通过三种多酚(单宁酸(TA)、茶多酚(TP)和花青素(ACN))与铁(III)涂层相结合制备的金属-酚网络(MPNs)在模拟消化和储存过程中对其的保护效果。结果表明,MPNs在细菌表面形成了一层保护膜。虽然TA和ACN抑制了YJ7的生长,但TP促进了其增殖。在MPNs系统中,只有铁(III)-TA表现出生长抑制作用。值得注意的是,ACN在最初2小时内增殖率最高,其次是3至4小时的TP。所有MPN涂层组在25℃和-20℃下均保持较高的细菌活力,其中TP涂层的细菌活细胞数最高,其次是TA和ACN。体外消化实验进一步表明,铁(III)-ACN组对人工胃液的抵抗力最强。总之,茶多酚和花青素在益生菌包封方面显示出卓越的潜力,在消化过程中提供保护稳定性,并在储存条件下提高活力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46cc/12248584/192287580079/foods-14-02277-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46cc/12248584/c409fd307fe9/foods-14-02277-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46cc/12248584/0395a6c157be/foods-14-02277-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46cc/12248584/b70e2d3845b2/foods-14-02277-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46cc/12248584/1977c63f0546/foods-14-02277-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46cc/12248584/8ac3ed3d59a1/foods-14-02277-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46cc/12248584/192287580079/foods-14-02277-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46cc/12248584/c409fd307fe9/foods-14-02277-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46cc/12248584/0395a6c157be/foods-14-02277-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46cc/12248584/b70e2d3845b2/foods-14-02277-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46cc/12248584/1977c63f0546/foods-14-02277-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46cc/12248584/8ac3ed3d59a1/foods-14-02277-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46cc/12248584/192287580079/foods-14-02277-g006.jpg

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本文引用的文献

[1]
Unveiling the Potential of Single-Cell Encapsulation in Biomedical Applications: Current Advances and Future Perspectives.

Small Sci. 2024-3-25

[2]
The Encapsulation Strategies for Targeted Delivery of Probiotics in Preventing and Treating Colorectal Cancer: A Review.

Adv Sci (Weinh). 2025-5

[3]
Metal-phenolic networks enhanced the protection of excipients for probiotics during freeze-drying.

Food Res Int. 2025-4

[4]
Metal-phenolic network biointerface-mediated cell regulation for bone tissue regeneration.

Mater Today Bio. 2024-12-12

[5]
Effects of nanoparticle size, shape, and zeta potential on drug delivery.

Int J Pharm. 2024-12-5

[6]
Single-cell encapsulation systems for probiotic delivery: Armor probiotics.

Adv Colloid Interface Sci. 2024-10

[7]
Recent Advances in the Understanding of Stress Resistance Mechanisms in Probiotics: Relevance for the Design of Functional Food Systems.

Probiotics Antimicrob Proteins. 2025-2

[8]
attenuates glucocorticoid-induced osteoporosis by altering the composition of rat gut microbiota and serum metabolic profile.

Front Immunol. 2023

[9]
Metal-Phenolic Network Directed Coating of Single Probiotic Cell Followed by Photoinitiated Thiol-Ene Click Fortification to Enhance Oral Therapy.

Small. 2024-5

[10]
Lactobacillus Plantarum NC8 and its metabolite acetate alleviate type 1 diabetes via inhibiting NLRP3.

Microb Pathog. 2023-9

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