Zhang Haoxuan, Zhang Huange, Zhong Hao
College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China.
Foods. 2025 Jun 26;14(13):2277. doi: 10.3390/foods14132277.
exhibits probiotic effects, including regulating the balance of the intestinal microbiota and enhancing immune function. However, this strain often experiences viability loss upon ingestion due to harsh conditions within the human digestive tract. This study aimed to evaluate the efficacy of metal-phenol networks (MPNs) fabricated via three polyphenols-tannic acid (TA), tea polyphenol (TP), and anthocyanin (ACN)-combined with Fe(III) coatings in protecting during simulated digestion and storage. The results demonstrated that MPNs formed a protective film on the bacterial surface. While TA and ACN inhibited the growth of YJ7, TP stimulated proliferation. Within the MPNs system, only Fe(III)-TA exhibited growth-inhibitory effects. Notably, ACN displayed the highest proliferation rate during the initial 2 h, followed by TP between 3 and 4 h. All MPN-coated groups maintained high bacterial viability at 25 °C and -20 °C, with TP-coated bacteria showing the highest viable cell count, followed by TA and ACN. In vitro digestion experiments further revealed that the Fe(III)-ACN group exhibited the strongest resistance to artificial gastric juice. In conclusion, tea polyphenol and anthocyanin demonstrate superior potential for probiotic encapsulation, offering both protective stability during digestion and enhanced viability under storage conditions.
具有益生菌作用，包括调节肠道微生物群平衡和增强免疫功能。然而，由于人类消化道内的恶劣条件，这种菌株在摄入后常常会出现活力丧失的情况。本研究旨在评估通过三种多酚（单宁酸（TA）、茶多酚（TP）和花青素（ACN））与铁（III）涂层相结合制备的金属-酚网络（MPNs）在模拟消化和储存过程中对其的保护效果。结果表明，MPNs在细菌表面形成了一层保护膜。虽然TA和ACN抑制了YJ7的生长，但TP促进了其增殖。在MPNs系统中，只有铁（III）-TA表现出生长抑制作用。值得注意的是，ACN在最初2小时内增殖率最高，其次是3至4小时的TP。所有MPN涂层组在25℃和-20℃下均保持较高的细菌活力，其中TP涂层的细菌活细胞数最高，其次是TA和ACN。体外消化实验进一步表明，铁（III）-ACN组对人工胃液的抵抗力最强。总之，茶多酚和花青素在益生菌包封方面显示出卓越的潜力，在消化过程中提供保护稳定性，并在储存条件下提高活力。
