von Kiel K, Hameister H, Somssich I E, Adolph S
Chromosoma. 1985;93(1):69-76. doi: 10.1007/BF01259448.
One of the best documented autosomal linkage associations in man is on chromosome 1p and in the mouse on chromosome 4. On mitotic chromosomes this genetic homology is shown more clearly by early replication banding (RBG; induced by incorporation of 5'bromodeoxyuridine (BrdU) in the second half of the S phase) than by structural banding (induced on prefixed chromosomes by denaturation, RFA, or trypsin, GTG). To analyse this phenomenon in more detail, 11 chromosomal regions in man and the domestic cat with known genetic homology were compared. In four chromosome pairs RBG and GTG banding show the same degree of homology. In seven chromosome pairs the homology is more pronounced by RBG than by GTG banding. RFA banding does not reveal the same extent of homology as does RBG banding. These results clearly show a difference between the structural banding pattern, RFA and GTG, and the replication banding pattern, RBG. The following conclusions can be drawn: in chromosomal regions with homologous functions the DNA replicates in the same temporal order. Early replication banding (RBG) reveals a functional pattern in these regions which has been more strongly preserved during evolution than the underlying chromosomal DNA. Differences in chromosomal banding are most prominent in the GTG banding pattern, whereas similarities are most apparent in the RBG banding pattern.
人类中记录最完善的常染色体连锁关联之一位于1号染色体上，而在小鼠中则位于4号染色体上。在有丝分裂染色体上，这种遗传同源性通过早期复制带型（RBG；由在S期后半段掺入5-溴脱氧尿苷（BrdU）诱导）比通过结构带型（由变性、RFA或胰蛋白酶在预处理染色体上诱导，GTG）更清楚地显示出来。为了更详细地分析这种现象，对人类和家猫中11个已知遗传同源性的染色体区域进行了比较。在四对染色体中，RBG和GTG带型显示出相同程度的同源性。在七对染色体中，RBG比GTG带型的同源性更明显。RFA带型没有显示出与RBG带型相同程度的同源性。这些结果清楚地表明了结构带型模式（RFA和GTG）与复制带型模式（RBG）之间的差异。可以得出以下结论：在具有同源功能的染色体区域，DNA以相同的时间顺序复制。早期复制带型（RBG）揭示了这些区域中的一种功能模式，这种模式在进化过程中比潜在的染色体DNA保存得更强烈。染色体带型的差异在GTG带型模式中最为突出，而相似性在RBG带型模式中最为明显。
