褪黑素调控的乙烯信号基因CpG岛中的DNA甲基化谱变化参与减轻采后番茄果实的冷害

DNA Methylation Profile Changes in CpG Islands of Ethylene-Signaling Genes Regulated by Melatonin Were Involved in Alleviating Chilling Injury of Postharvest Tomato Fruit.

作者信息

Yan Jingrui, Shan Shuangshuang, Li Jiangkuo, Zhang Zhengke, Yang Jiali, Zhang Wanli, Song Hongmiao, Xu Xiangbin, Duan Wenhui

机构信息

College of Food Science and Engineering, Hainan University, Haikou 570228, China.

Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products, National Engineering and Technology Research Center for Preservation of Agricultural Products, Tianjin 300384, China.

出版信息

Int J Mol Sci. 2025 Jun 26;26(13):6170. doi: 10.3390/ijms26136170.

DOI:10.3390/ijms26136170

PMID:40649950

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12249976/

Abstract

Melatonin (MT) has been reported to alleviate chilling injury (CI) in postharvest tomato fruit during low-temperature storage. In the present study, the DNA methylation profile changes in the CpG islands of ethylene signaling genes regulated by MT in postharvest tomato fruit during low-temperature storage were detected. The MT treatment increased the content of total soluble solids (TSS) and enhanced the ethylene production of tomato fruit. Moreover, it decreased titratable acidity (TA) content, inhibited the activity of polygalacturonase (PG), and kept the firmness of tomato fruit under low-temperature storage. In the MT-treated tomato fruit, significant changes in DNA methylation of CpG island of , , , , and genes were induced; the expression of was inhibited; and the expression of , , and genes was increased, by which the ethylene signaling might be influenced and the CI was alleviated. The present results provide evidence that the CI of postharvest tomato fruit alleviated by MT might be related to the changes in DNA methylation of ethylene-signaling genes.

摘要

据报道，褪黑素（MT）可减轻采后番茄果实低温贮藏期间的冷害（CI）。在本研究中，检测了采后番茄果实低温贮藏期间MT调控的乙烯信号基因CpG岛中的DNA甲基化谱变化。MT处理增加了总可溶性固形物（TSS）含量，增强了番茄果实的乙烯生成。此外，它降低了可滴定酸度（TA）含量，抑制了多聚半乳糖醛酸酶（PG）的活性，并在低温贮藏下保持了番茄果实的硬度。在MT处理的番茄果实中，诱导了、、、和基因CpG岛DNA甲基化的显著变化；的表达受到抑制；、和基因的表达增加，由此可能影响乙烯信号传导并减轻冷害。目前的结果提供了证据，表明MT减轻采后番茄果实冷害可能与乙烯信号基因DNA甲基化的变化有关。