Totè Chiara Maria, Capponi Martina, Salvatori Francesca, Zicari Anna Maria, Guido Cristiana Alessia, Brindisi Giulia, Iantorno Laura, Bartimoccia Simona, Baratta Francesco, Forte Maurizio, Picchio Vittorio, Malvasi Mariaelena, Aloisio Simone, Pacella Elena, Pignatelli Pasquale, Violi Francesco, Carnevale Roberto, Spalice Alberto, Loffredo Lorenzo
Department of Clinical Internal, Anaesthetic and Cardiovascular Science, Sapienza University of Rome, Viale del Policlinico, 155, 00161 Rome, RM, Italy.
Department of Mother-Child, Urological Science, La Sapienza University, 00161 Rome, RM, Italy.
Int J Mol Sci. 2025 Jul 1;26(13):6336. doi: 10.3390/ijms26136336.
Pediatric Autoimmune Neuropsychiatric Disorders Associated with Streptococcal Infections (PANDAS), unlike pediatric acute-onset neuropsychiatric syndrome (PANS), is triggered by infections. This study aimed to assess the differences in low-grade endotoxemia and oxidative stress between these conditions. A cross-sectional study compared serum levels of soluble NOX2-dp (sNOX-2-dp), isoprostanes, lipopolysaccharide (LPS), and zonulin in 30 PANDAS, 21 PANS, and 30 control (CT) children matched for age and gender. Zonulin was used to assess gut permeability. Patients with PANDAS showed significantly higher serum levels of sNOX2-dp, isoprostanes, LPS, and zonulin than PANS and controls, while no significant differences were found between PANS and controls. sNOX2-dp correlated with isoprostanes (Rs = 0.708; < 0.001), LPS (Rs = 0.584; < 0.001), and zonulin (Rs = 0.662; < 0.001). Multiple regression identified isoprostanes (β = 0.599; < 0.001) and zonulin (β = 0.295; = 0.01) as independent predictors of sNOX2-dp (R = 81%). PANDAS and PANS showed distinct profiles of LPS, zonulin, NOX2, and isoprostanes. Future research should explore therapies targeting endotoxemia and oxidative stress for potential clinical benefits.
与链球菌感染相关的小儿自身免疫性神经精神障碍（PANDAS），与小儿急性起病神经精神综合征（PANS）不同，是由感染引发的。本研究旨在评估这些病症在低度内毒素血症和氧化应激方面的差异。一项横断面研究比较了30名PANDAS患儿、21名PANS患儿和30名年龄及性别匹配的对照（CT）儿童的血清可溶性NOX2 - dp（sNOX - 2 - dp）、异前列腺素、脂多糖（LPS）和闭合蛋白水平。闭合蛋白用于评估肠道通透性。PANDAS患儿的血清sNOX2 - dp、异前列腺素、LPS和闭合蛋白水平显著高于PANS患儿和对照组，而PANS患儿和对照组之间未发现显著差异。sNOX2 - dp与异前列腺素（Rs = 0.708；<0.001）、LPS（Rs = 0.584；<0.001）和闭合蛋白（Rs = 0.662；<0.001）相关。多元回归分析确定异前列腺素（β = 0.599；<0.001）和闭合蛋白（β = 0.295；= 0.01）是sNOX2 - dp的独立预测因子（R = 81%）。PANDAS和PANS在LPS、闭合蛋白、NOX2和异前列腺素方面表现出不同的特征。未来的研究应探索针对内毒素血症和氧化应激的治疗方法，以获得潜在的临床益处。
