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软组织中DNA链断裂的快速分析

Rapid analysis of DNA strand breaks in soft tissues.

作者信息

Morris S R, Shertzer H G

出版信息

Environ Mutagen. 1985;7(6):871-80. doi: 10.1002/em.2860070608.

DOI:10.1002/em.2860070608
PMID:4065061
Abstract

A technique was developed to measure rapidly DNA strand breaks in soft tissues. This method measured the rate of alkaline unwinding of DNA, which was proportional to strand breakage. Alkaline unwinding of DNA was done by treating tissue homogenates with NaOH. Single-stranded DNA was removed by extraction with aqueous phenol. DNA unwinding was quantitated by measuring the remaining double-stranded DNA. Using the described technique, a dose-effect relationship was observed between N-nitrosodimethylamine (NDMA) and alkaline unwinding of mouse liver DNA.

摘要

开发了一种用于快速测量软组织中DNA链断裂的技术。该方法测量DNA的碱性解旋速率，其与链断裂成正比。DNA的碱性解旋通过用NaOH处理组织匀浆来完成。用酚水溶液萃取去除单链DNA。通过测量剩余的双链DNA对DNA解旋进行定量。使用所描述的技术，观察到N-亚硝基二甲胺（NDMA）与小鼠肝脏DNA碱性解旋之间的剂量效应关系。

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