Elbeltagi Shehab, Al-Zharani Mohammed, Nasr Fahd A, Ismail A M, El-Tohamy Hagar M, Abdelbased Khaled M, Eldin Zienab E, Al-Theyab Nada S
Department of Physics, Faculty of Science, New Valley University, El-Kharga 72511, Egypt.
Biology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia.
Colloids Surf B Biointerfaces. 2025 Nov;255:114940. doi: 10.1016/j.colsurfb.2025.114940. Epub 2025 Jul 9.
Cellmembrane-coated nanoparticles (NPs) have garnered significant attention because of their extended circulation time and ability to target similar cells through homotypic interactions. Herein, we fabricated cancer cell membrane (CCM)-coated quercetin (Q)-MXene-Au NPs, forming Q-MX-Au@CCM, as a promising approach for drug delivery (DD) and improved photothermal therapy (PTT). The Q-MX-Au@CCM nanocomposites serve as multifunctional nanoplatforms with a large surface area and excellent responsiveness to near-infrared (NIR) light thereby enhancing their efficacy in tumor treatment. TEM analysis revealed that Q-MX-Au@CCM had an average particle size of 87.4 nm and a zeta potential of -28.9 mV. MXene-Au demonstrated excellent PT properties, achieving a PT conversion efficiency of 68.5 %. The MTT assay demonstrated significant cytotoxicity of MCF-7 breast cancer cells, with the chemo-PTT approach showing an IC value of 12.5 µg/mL and resulting a high apoptosis rate. Cellular internalization studies showed enhanced uptake of both MX-Au and Q-MX-Au@CCM NPs in MCF-7 cells under NIR. The pro-apoptotic efficacy of Q and Q-MX-Au@CCM was further confirmed with Western blot analysis of key apoptotic markers, Bax and BCl-2. Q-MX-Au@CCM showed the most pronounced effects, significantly increasing Bax expression (0.75 ± 0.06) and decreasing BCl-2 expression (0.27 ± 0.011). In vivo studies using a xenograft model in BALB/c mice indicated that chemo-PTT resulted in the lowest tumor weight (0.08 g) and volume (46.3 mm). Molecular docking studies showed strong binding affinity between MXene and Adora2a (binding energy: -12.9 kcal/mol), with key interactions including two hydrogen bonds (HB): one between the oxygen of Q and HIS273 (2.94 Å), and another between the HB of Q and the oxygen of ALA78 (2.46 Å), supporting the molecular basis for the enhanced therapeutic performance of Q-MX-Au@CCM.
细胞膜包覆的纳米颗粒（NPs）因其延长的循环时间以及通过同型相互作用靶向相似细胞的能力而备受关注。在此，我们制备了癌细胞膜（CCM）包覆的槲皮素（Q）-MXene-金纳米颗粒，即Q-MX-Au@CCM，作为一种有前景的药物递送（DD）和改进光热疗法（PTT）的方法。Q-MX-Au@CCM纳米复合材料作为多功能纳米平台，具有大的表面积和对近红外（NIR）光的优异响应性，从而提高了它们在肿瘤治疗中的疗效。透射电子显微镜（TEM）分析表明，Q-MX-Au@CCM的平均粒径为87.4nm，zeta电位为-28.9mV。MXene-金表现出优异的光热性能，实现了68.5%的光热转换效率。MTT试验证明了MCF-7乳腺癌细胞具有显著的细胞毒性，化学-光热疗法的IC值为12.5μg/mL，导致高凋亡率。细胞内化研究表明，在近红外光下，MCF-7细胞对MX-金和Q-MX-Au@CCM纳米颗粒的摄取增强。通过对关键凋亡标志物Bax和BCl-2的蛋白质印迹分析，进一步证实了Q和Q-MX-Au@CCM的促凋亡功效。Q-MX-Au@CCM表现出最显著的效果，显著增加Bax表达（0.75±0.06）并降低BCl-2表达（0.27±0.011））。在BALB/c小鼠中使用异种移植模型进行的体内研究表明，化学-光热疗法导致最低的肿瘤重量（0.08g）和体积（46.3mm）。分子对接研究表明MXene与Adora2a之间具有很强的结合亲和力（结合能：-12.9kcal/mol），关键相互作用包括两个氢键（HB）：一个在Q的氧与HIS273之间（2.94Å），另一个在Q的HB与ALA78的氧之间（2.46Å），支持了Q-MX-Au@CCM增强治疗性能的分子基础。
