Farsi J M, Sodek J, Aubin J E
Exp Cell Res. 1985 Dec;161(2):473-83. doi: 10.1016/0014-4827(85)90102-8.
We have studied the ability of human gingival fibroblasts (HGF) to attach to different interstitial (types I, II and III) and basement membrane (types IV and V) collagens. HGF cells were plated onto collagen-coated Petri dishes under various conditions and the percentage of cells attaching to the collagen was determined. HGF were found to attach to all the different types of native collagens, but attached poorly to the corresponding denatured collagens. When plated in the presence of 15% fetal bovine serum (FBS) or fibronectin-depleted FBS, similar percentages (approximately 85%) of cells attached to both interstitial and basement membrane collagens, demonstrating an attachment mechanism that is independent of plasma fibronectin. That the attachment in the presence of serum was also independent of cellular fibronectin was shown by the inability of fibronectin antibodies to block attachment to any of the collagen types. HGF were also capable of attaching to all of the collagen types in the complete absence of serum. In previous studies, investigators using cell lines have suggested that cell attachment in the absence of serum is non-physiological. However, the serum-free attachment of HGF to collagen was found to be dependent on cellular protein synthesis indicating that this attachment mechanism has biological significance.
我们研究了人牙龈成纤维细胞（HGF）附着于不同的间质胶原（I型、II型和III型）和基底膜胶原（IV型和V型）的能力。在各种条件下，将HGF细胞接种到包被有胶原的培养皿上，然后测定附着于胶原的细胞百分比。结果发现，HGF能附着于所有不同类型的天然胶原，但对相应的变性胶原附着较差。当在15%胎牛血清（FBS）或去除纤连蛋白的FBS存在下接种时，附着于间质胶原和基底膜胶原的细胞百分比相似（约85%），这表明存在一种不依赖血浆纤连蛋白的附着机制。纤连蛋白抗体无法阻断对任何一种胶原类型的附着，这表明在血清存在下的附着也不依赖细胞纤连蛋白。在完全无血清的情况下，HGF也能够附着于所有类型的胶原。在先前的研究中，使用细胞系的研究者认为无血清时的细胞附着是非生理性的。然而，发现HGF在无血清情况下对胶原的附着依赖于细胞蛋白质合成，这表明这种附着机制具有生物学意义。
