Scott D M, Murray J C, Barnes M J
Exp Cell Res. 1983 Apr 1;144(2):472-8. doi: 10.1016/0014-4827(83)90427-5.
Bovine corneal endothelial cells adhered equally well to a variety of collagens (types I, III, IV and V) consistent with a role for fibronectin in this process. They did not exhibit a preferential binding to collagen type IV--as might be anticipated if laminin were to play a significant role in their adhesion. Inhibition studies with anti-fibronectin antibodies demonstrated the importance of endogenous fibronectin in the mediation of attachment. Consistent with this, binding did not appear to require the presence of exogenous protein, since cells bound to collagens equally well in the presence or absence of added fibronectin and binding was not stimulated by pretreatment of collagens with this protein.
牛角膜内皮细胞与多种胶原蛋白（I型、III型、IV型和V型）的黏附效果相同，这与纤连蛋白在此过程中的作用一致。它们并未表现出对IV型胶原蛋白的优先结合——如果层粘连蛋白在其黏附中起重要作用，情况可能会有所不同。用抗纤连蛋白抗体进行的抑制研究表明内源性纤连蛋白在介导细胞黏附方面的重要性。与此相符的是，黏附似乎并不需要外源蛋白的存在，因为无论有无添加纤连蛋白，细胞与胶原蛋白的黏附效果均相同，且用该蛋白预处理胶原蛋白并不能刺激黏附。
