Zhang Yulong, Li Xingwei, Chen Hongao, Xie Xianrui, Li Chengbo, Hou Guige, Ding Yuexia, Gao Zhongfei
School of Pharmacy, Key Laboratory of Medical Antibacterial Materials of Shandong Province, Binzhou Medical University, Yantai 264003, PR China.
School of Pharmacy, Key Laboratory of Medical Antibacterial Materials of Shandong Province, Binzhou Medical University, Yantai 264003, PR China; Department of Pharmacy, Yantai Yuhuangding Hospital, Yantai 264000, PR China.
Int J Biol Macromol. 2025 Aug;320(Pt 2):145944. doi: 10.1016/j.ijbiomac.2025.145944. Epub 2025 Jul 11.
Mammalian skin is composed of hydrophobic polymers such as proteins, polysaccharide and oily matrices, serving as a crucial barrier to prevent adverse factors from rapidly infiltrating the body while also functioning as a key organ for material exchange. However, open wounds resulting from skin defects can disrupt the internal stability of the body's environment, leading to water loss and delaying the wound healing process. Mannan is believed to synergize with natural moisturizing factors (NMF). Inspired by the hydrophobic structure of skin, firstly, acetylated yeast mannans (AM) were synthetized as a hydrophobic and moisturizing component. Subsequently, we developed a composite electrospun nanofiber (PVCQAM), which integrates polyvinyl alcohol (PVA, a biocompatible component), AM, collagen (Col, extracellular matrix component), and quaternized chitosan (QCS, a hydrophobic and antibacterial component) to the mimic the architecture and functions involved in wound healing. AM enhanced the surface hydrophobicity compared to the mannan-containing nanofibers (PVCQM). Furthermore, as the degree of acetylation increased, nanofibers manifested improved hydrophobic properties. The nanofibers demonstrated thermal stability, and suitable stretchability that aligns with human tissue characteristics. The incorporation of QCS imparted inherent antibacterial effects to the biomimetic nanofibers against S. aureus and E. coli. Notably, excellent cytocompatibility and hemocompatibility in vitro have prompted us to evaluate the therapeutic efficacy in a cutaneous defect model. The electrospun nanofibers in this study displayed significant anti-inflammatory effects and promoted collagen deposition, resulting in an impressive wound closure rate of 95 %. The skin-inspired nanofiber dressing based on hydrophobic AM presents significant potential for moisturizing and facilitating benign tissue repair in defect management.
哺乳动物的皮肤由蛋白质、多糖和油性基质等疏水性聚合物组成，它是一道至关重要的屏障，可防止不利因素迅速侵入体内，同时也是物质交换的关键器官。然而，皮肤缺损造成的开放性伤口会破坏身体内环境的稳定性，导致水分流失并延缓伤口愈合过程。甘露聚糖被认为能与天然保湿因子（NMF）协同作用。受皮肤疏水结构的启发，首先合成了乙酰化酵母甘露聚糖（AM）作为疏水保湿成分。随后，我们开发了一种复合电纺纳米纤维（PVCQAM），它整合了聚乙烯醇（PVA，一种生物相容性成分）、AM、胶原蛋白（Col，细胞外基质成分）和季铵化壳聚糖（QCS，一种疏水抗菌成分），以模拟伤口愈合过程中涉及的结构和功能。与含甘露聚糖的纳米纤维（PVCQM）相比，AM增强了表面疏水性。此外，随着乙酰化程度的增加，纳米纤维的疏水性能得到改善。这些纳米纤维具有热稳定性和与人体组织特性相匹配的合适拉伸性。QCS的加入赋予了仿生纳米纤维对金黄色葡萄球菌和大肠杆菌的固有抗菌作用。值得注意的是，体外出色的细胞相容性和血液相容性促使我们在皮肤缺损模型中评估其治疗效果。本研究中的电纺纳米纤维显示出显著的抗炎作用并促进了胶原蛋白沉积，伤口闭合率高达95%，令人印象深刻。基于疏水AM的皮肤启发式纳米纤维敷料在缺损管理中具有显著的保湿和促进良性组织修复的潜力。
