通过核磁共振确定细胞内水空间。

The determination of intracellular water space by NMR.

作者信息

Shinar H, Navon G

出版信息

FEBS Lett. 1985 Nov 25;193(1):75-8. doi: 10.1016/0014-5793(85)80082-x.

Abstract

A new method for the determination of intracellular water space using NMR spectroscopy is described. The method is based on the measurement of 59Co NMR signal intensity of an inert, stable and membrane-impermeable cobalt(III) compound such as Co(CN)3-6 or Co(imidazole)3+6 and the 2H or 1H NMR signal intensities of the freely permeable water. As an example of the method, the variation of the intracellular water space of human erythrocytes as a function of osmolality was measured.

摘要

描述了一种使用核磁共振光谱法测定细胞内水空间的新方法。该方法基于测量惰性、稳定且不可透过细胞膜的钴（III）化合物（如六氰合钴（III）酸根离子或六咪唑合钴（III）离子）的59Co核磁共振信号强度以及可自由透过的水的2H或1H核磁共振信号强度。作为该方法的一个例子，测量了人类红细胞内水空间随渗透压的变化。