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有核红细胞和细胞核悬液中的钠-23核磁共振弛豫时间。

Sodium-23 NMR relaxation times in nucleated red blood cells and suspensions of nuclei.

作者信息

Shinar H, Navon G

机构信息

Raymond and Beverly Sackler Faculty of Exact Sciences, School of Chemistry, Tel-Aviv University, Ramat Aviv, Israel.

出版信息

Biophys J. 1991 Jan;59(1):203-8. doi: 10.1016/S0006-3495(91)82211-1.

Abstract

The relaxation behavior of intracellular 23Na in suspensions of chicken erythrocytes and of their nuclei was investigated. The transverse magnetization was found to decay biexponentially. The average relaxation rates for the nucleated chicken erythrocytes are considerably shorter than the average relaxation rates obtained for dog and human nonnucleated red blood cells. Of particular significance is the twofold decrease in the short component of T2. Calculations based on the measured 23Na NMR relaxation rates in suspensions of nuclei indicate that most of the difference between the relaxation rates in the mammalian as compared to the chicken erythrocytes, can be accounted for by the contribution of the nuclei in the latter.

摘要

研究了鸡红细胞及其细胞核悬浮液中细胞内23Na的弛豫行为。发现横向磁化强度呈双指数衰减。有核鸡红细胞的平均弛豫率明显短于狗和人类无核红细胞的平均弛豫率。特别重要的是T2短成分下降了两倍。基于测得的细胞核悬浮液中23Na NMR弛豫率的计算表明,与鸡红细胞相比,哺乳动物弛豫率之间的大部分差异可归因于后者中细胞核的贡献。

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本文引用的文献

5
On the attachment of the nuclear pore complex.关于核孔复合体的附着。
J Cell Biol. 1974 Sep;62(3):746-54. doi: 10.1083/jcb.62.3.746.
6
The determination of intracellular water space by NMR.通过核磁共振确定细胞内水空间。
FEBS Lett. 1985 Nov 25;193(1):75-8. doi: 10.1016/0014-5793(85)80082-x.

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